July Week1

Plasmid Amplification for further construction; chose pRSFDuet-1, pACYCDuet-1 and pCDFDuet-1 for expression vector and pBluescript II KS（+） for connector.

July Week2

Construct the gene of our fusion protein: ssDsbA-FP-HL-Lgt-FL-TAL using splicing by overlap extension. We connected ssDsbA-FP-HL-lgt for one part of this fusion protein and FL-TAL for another. Then connect them together.

July Week3

Connected ssDsbA-FP-HL-lgt by splicing by overlap extension. Transform, colony picking plasmid extraction and digestion identification; Sequencing results showed accurate construction.

July Week4

Use golden gate to connected TAL of 2012 Freiburg igem. Transform, colony picking plasmid extraction and digestion identification.