Team:SJTU-BioX-Shanghai/daynotes

From 2014.igem.org

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                    <h2 id ="JulyWeek1" >July Week1</h2>
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                    <p>Plasmid Amplification for further construction; chose pRSFDuet-1, pACYCDuet-1 and pCDFDuet-1 for expression vector and pBluescript II KS（+） for connector.<p>
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                    <h2 id ="JulyWeek2" >July Week2</h2>
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                    <p>Construct the gene of our fusion protein: ssDsbA-FP-HL-Lgt-FL-TAL using splicing by overlap extension.  We connected ssDsbA-FP-HL-lgt for one part of this fusion protein and FL-TAL for another. Then connect them together.<p>
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                    <h2 id ="JulyWeek3" >July Week3</h2>
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                    <p> Connected ssDsbA-FP-HL-lgt by splicing by overlap extension. Transform, colony picking plasmid extraction and digestion identification; Sequencing results showed accurate construction.<p>
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                    <h2 id ="JulyWeek4" >July Week4</h2>
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                    <p> Use golden gate to connected TAL of 2012 Freiburg igem. Transform, colony picking plasmid extraction and digestion identification.<p>                    
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Revision as of 13:00, 12 October 2014

July Week1

Plasmid Amplification for further construction; chose pRSFDuet-1, pACYCDuet-1 and pCDFDuet-1 for expression vector and pBluescript II KS（+） for connector.

July Week2

Construct the gene of our fusion protein: ssDsbA-FP-HL-Lgt-FL-TAL using splicing by overlap extension. We connected ssDsbA-FP-HL-lgt for one part of this fusion protein and FL-TAL for another. Then connect them together.

July Week3

Connected ssDsbA-FP-HL-lgt by splicing by overlap extension. Transform, colony picking plasmid extraction and digestion identification; Sequencing results showed accurate construction.

July Week4

Use golden gate to connected TAL of 2012 Freiburg igem. Transform, colony picking plasmid extraction and digestion identification.

Bio-X Institutes

Shanghai Jiao Tong University

800, Dongchuan Road 200240, Shanghai, China

sunyaojin2011@foxmail.com

Retrieved from "http://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes"