Team:SJTU-BioX-Shanghai/Results

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Revision as of 16:18, 17 October 2014

Reformed Plasmid Result

We designed four reformed plasmids, pBluescript II KS(+) ScaI deletion, pBluescript II KS(+) EcoRV deletion, pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy. And we test their co-transformation ability and get the result picture. All of these plasmid has Amp antibiotic resistance.
We co-transformed pRSFDuet-1 and these four plasmids, respectively. So the plate has two antibiotic resistance, Kan and Amp. We culture 4 different strains on the plate and get the result picture. Figure 2.1.1 Four plates of Co-transfected bacteria So we experimentally validate that our reformed plasmids perform as expected.

@@ Line 17: / Line 17: @@
 <h2>Reformed Plasmid Result</h2>
 We designed four reformed plasmids, pBluescript II KS(+) ScaI deletion, pBluescript II KS(+) EcoRV deletion, pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy.
-And we test their co-transformation ability and get the result picture. All of these plasmid has Amp antibiotic resistance.
+And we test their co-transformation ability and get the result picture. All of these plasmid has Amp antibiotic resistance. <br>
+We co-transformed pRSFDuet-1 and these four plasmids, respectively. So the plate has two antibiotic resistance, Kan and Amp. We culture 4 different strains on the plate and get the result picture.
 <center><img src="" width=600px></img></center>
-Figure 2.1.1 Four plate of Co-transfected bacteria
+Figure 2.1.1 Four plates of Co-transfected bacteria
 So we experimentally validate that our reformed plasmids perform as expected.