Team:SJTU-BioX-Shanghai

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<div id="content1" class="first">
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<img class="front" src="http://2014.igem.org/wiki/images/3/30/Front-1.png"></img>
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<p>Hi, everyone!</p>
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<p>There is a Chinese song named</p>
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<p><strong id="light" class="big">"Unity is the strength."</strong></p>
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<p>This year, we want to explore this great power.</p>
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    <div id="content2" class="first">
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<img class="front" src="http://2014.igem.org/wiki/images/6/64/Front-2.png"></img>
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<p>Of course, we are not talking about ourselves.</p>
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<p>The main characters are...</p>
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<p><strong id="light" class="big">Enzymes!!</strong></p>
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<p>Do you know that the more enzymes <strong id="light" class="small">polymerize</strong>,</p>
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<p>the more useful they become?</p>
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<p>Enzymes couldn't get close to each others easily.</p>
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<p>Therefore,</p>
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<p>we help them using a structure we call</p>
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<p><a href="/Team:SJTU-BioX-Shanghai/Part1_Connect"> <strong id="blue" class="big"> "Crown"</strong> </a></p>
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<p> to connect them together.</p>
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    <div id="content4" class="first">
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<img class="front" src="http://2014.igem.org/wiki/images/4/43/Front-4.png"></img>
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<p>That's not all.</p>
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<p>We try to improve our idea by taking </p>
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<a href="/Team:SJTU-BioX-Shanghai/Part2_Extension"><p><strong id="light" class="big">Polymerization</strong></p>
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<p>and</p>
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<p><strong id="light" class="big">Selectivity</strong></p></a>
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<p>into account.</p>
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<img class="front" src="http://2014.igem.org/wiki/images/2/2e/Front-5.png"></img>  
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<p>Oops, am I talking too much?</p>
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<p>Alright, I will just let you figure out by yourselves.</p>
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<p>At last, thanks for reading our <strong id="light" class="big">wiki</strong>~</p>
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<a href="http://2014.igem.org/Team:SJTU-BioX-Shanghai"style="color:#000000">Home </a> </td>
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    <center><h5>Want to have a quick view?</h5></center>
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<a href="http://2014.igem.org/Team:SJTU-BioX-Shanghai/Project"style="color:#000000"> Project</a></td>
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      <a href="/Team:SJTU-BioX-Shanghai/Results" title="RESULTS">
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    <a href="/Team:SJTU-BioX-Shanghai/Modeling" title="MODELING">
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<p>This year, we bring MembRing – an annular protein multimerization system in cytomembrane.</p>
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<p>This system will help us achieve the goal of selective polymerization of enzymes. Polymerized enzymes, different from normal scattered condition, has much more opportunities to contact with the substrates. Therefore, our project aims to increase as well as control the efficiency of complex reactions.</p>
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<p>The basic function, polymerization, is carried out by a circular DNA and DNA-binding protein to polymerize enzymes. First, a fluorescent protein, a transmembrane domain, an enzyme and a DNA binding protein are connected in order, forming a complex that the FP locates outside the film while the enzyme and DNA binding protein are in cytoplasm. Then integrate specific matching DNA sequences onto an exogenous plasmid and co-transform this connecter with expression vectors into E.Coli to associate enzymes.</p>
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<p>In addition, we plan to make the enzymes selectable by remolding the connecter with different recognition sequences and co-transforming it with expression vectors. This will help the whole project to work in all kinds of practical applications.
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Latest revision as of 02:09, 18 October 2014

Hi, everyone!

There is a Chinese song named

"Unity is the strength."

This year, we want to explore this great power.

Of course, we are not talking about ourselves.

The main characters are...

Enzymes!!

Do you know that the more enzymes polymerize,

the more useful they become?

Enzymes couldn't get close to each others easily.

Therefore,

we help them using a structure we call

"Crown"

to connect them together.

That's not all.

We try to improve our idea by taking

Polymerization

and

Selectivity

into account.

Oops, am I talking too much?

Alright, I will just let you figure out by yourselves.

At last, thanks for reading our wiki~