Team:SDU-Denmark/Tour45

From 2014.igem.org

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<i>Escherichia coli</i>, limonene synthase (LIMS1) from <i>Citrus limon</i>  
<i>Escherichia coli</i>, limonene synthase (LIMS1) from <i>Citrus limon</i>  
and appY from <i>Escherichia coli</i>.
and appY from <i>Escherichia coli</i>.
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Revision as of 14:41, 16 October 2014

Basic parts
BBa_K1475001
OneProt: The coding region of our self-designed nutritional protein. OneProt contains the recommended ratio of all the indispensible amino acids to a human diet. The protein is also compliant with the recommended ratio of indispensible to dispensable amino acids.

BBa_K1475002
fat-2: This part contains only the coding region of the fat-2 desaturase from Caenorhabditis elegans, the natural gene consists of three exons.

BBa_K1475003
TetR(N): the frequently used tet repressor from Escherichia coli (Part:BBa_C0040) modified by removing the LVA rapid degradation tag.
Regulatory devices
BBa_K1475004
Pcons-TetR(N)-term: the tet repressor from Escherichia coli is expressed form a constitutively active promoter. A terminator prevents transcription of genes downstream of the repressor.

BBa_K1475008
Pcon-LacI-term: Lac repressor protein derived from Escherichia coli without LVA degradation tag, controlled by a constitutively active promoter.

Constitutively active production devices
BBa_K1475000
p(tetR)-OneProt-term: OneProt is a self-designed protein designed to meet the recommended ratio of indispensible amino acids to a human diet according to the world health organization (WHO). It also contains the recommended ration of indispensible to dispensible amino acids. BBa_K1475009
P(TetR)-LIMS1-term: Limonene synthase from Citrus limon regulated by the tet promoter. BBa_K1475010
P(TetR)-dxs-LIMS1-appY-term: the limonene generator with tet promoter. The limonene generator consists of deoxyxylulose phosphtae synthase (dxs) from Escherichia coli, limonene synthase (LIMS1) from Citrus limon and appY from Escherichia coli.