http://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&feed=atom&action=historyTeam:SDU-Denmark/Tour43 - Revision history2024-03-29T10:42:45ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=397151&oldid=prevUlrikasimone at 03:27, 18 October 20142014-10-18T03:27:13Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:27, 18 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 3:</td>
<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Fatty acids</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Fatty acids</h3></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a class="popupImg alignRight" style="width:400px" target="_blank" href="https://static.igem.org/mediawiki/2014/5/53/2014SDUfattyacids2.png" title="Fish normaly provides a great amount of essential fatty acids."></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a class="popupImg alignRight" style="width:400px" target="_blank" href="https://static.igem.org/mediawiki/2014/5/53/2014SDUfattyacids2.png" title="Fish normaly provides a great amount of essential fatty acids."></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/9/92/2014SDUfattyacids1.png" style="width:400px" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/9/92/2014SDUfattyacids1.png" style="width:400px" /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fish normaly provides a great amount of essential fatty acids.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fish normaly provides a great amount of essential fatty acids.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">We wanted to clone</span> ∆9, ∆12 & ∆15 fatty acid desaturases into separate plasmids by the use of USER</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">We wanted to clone</span> ∆9, ∆12 & ∆15 fatty acid desaturases into separate plasmids by the use of USER</div></td></tr>
</table>Ulrikasimonehttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=396960&oldid=prevSarahNielsen at 03:26, 18 October 20142014-10-18T03:26:07Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:26, 18 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 5:</td>
<td colspan="2" class="diff-lineno">Line 5:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a class="popupImg alignRight" style="width:400px" target="_blank" href="https://static.igem.org/mediawiki/2014/5/53/2014SDUfattyacids2.png" title="Fish normaly provides a great amount of essential fatty acids."></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a class="popupImg alignRight" style="width:400px" target="_blank" href="https://static.igem.org/mediawiki/2014/5/53/2014SDUfattyacids2.png" title="Fish normaly provides a great amount of essential fatty acids."></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/9/92/2014SDUfattyacids1.png" style="width:400px" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/9/92/2014SDUfattyacids1.png" style="width:400px" /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Fish normaly provides a great amount of essential fatty acids</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Fish normaly provides a great amount of essential fatty acids<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
</table>SarahNielsenhttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=396913&oldid=prevSarahNielsen at 03:25, 18 October 20142014-10-18T03:25:53Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:25, 18 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 3:</td>
<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Fatty acids</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Fatty acids</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a class="popupImg alignRight" style="width:<del class="diffchange diffchange-inline">300px</del>" target="_blank" href="https://static.igem.org/mediawiki/2014/5/53/2014SDUfattyacids2.png" title="Fish normaly provides a great amount of essential fatty acids."></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><a class="popupImg alignRight" style="width:<ins class="diffchange diffchange-inline">400px</ins>" target="_blank" href="https://static.igem.org/mediawiki/2014/5/53/2014SDUfattyacids2.png" title="Fish normaly provides a great amount of essential fatty acids."></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/9/92/2014SDUfattyacids1.png" style="width:<del class="diffchange diffchange-inline">300px</del>" /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/9/92/2014SDUfattyacids1.png" style="width:<ins class="diffchange diffchange-inline">400px</ins>" /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fish normaly provides a great amount of essential fatty acids</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fish normaly provides a great amount of essential fatty acids</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></a></div></td></tr>
</table>SarahNielsenhttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=396862&oldid=prevSarahNielsen at 03:25, 18 October 20142014-10-18T03:25:35Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:25, 18 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 3:</td>
<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Fatty acids</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Fatty acids</h3></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a class="popupImg alignRight" style="width:300px" target="_blank" href="https://static.igem.org/mediawiki/2014/5/53/2014SDUfattyacids2.png" title="Fish normaly provides a great amount of essential fatty acids."></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/9/92/2014SDUfattyacids1.png" style="width:300px" /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Fish normaly provides a great amount of essential fatty acids</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></a></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>SarahNielsenhttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=370845&oldid=prevUlrikasimone at 23:51, 17 October 20142014-10-17T23:51:00Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:51, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 9:</td>
<td colspan="2" class="diff-lineno">Line 9:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning, we needed the desaturases separately.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning, we needed the desaturases separately.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="intro">We tried running several</span> <del class="diffchange diffchange-inline">USER PCRs </del>on ∆9 desaturase iGEM part but all were unsuccessful.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="intro">We tried running several <ins class="diffchange diffchange-inline">USER PCRs</ins></span> on ∆9 desaturase iGEM part but all were unsuccessful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="intro">We received the ∆12<del class="diffchange diffchange-inline"></span> </del>desaturase (FAT-2) which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which was successful. Thus we cloned it into a PSB1C3 plasmid and it can now be found in parts registry under </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="intro">We received the ∆12 desaturase (FAT-2)<ins class="diffchange diffchange-inline"></span> </ins>which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which was successful. Thus we cloned it into a PSB1C3 plasmid and it can now be found in parts registry under </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="intro">We tried to run</span> <del class="diffchange diffchange-inline">a colony PCR </del>on the bacterium <i>Synechocystis sp.</i> PCC6803 in order for us to get the ∆15</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="intro">We tried to run <ins class="diffchange diffchange-inline">a colony PCR</ins></span> on the bacterium <i>Synechocystis sp.</i> PCC6803<ins class="diffchange diffchange-inline">, with Phusion polymerase, </ins>in order for us to get the ∆15</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>desaturase. Unfortunately, this was unsuccessful.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>desaturase. Unfortunately, this was unsuccessful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Ulrikasimonehttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=307518&oldid=prevSarahNielsen at 07:21, 17 October 20142014-10-17T07:21:12Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 07:21, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 11:</td>
<td colspan="2" class="diff-lineno">Line 11:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">We tried running several</span> USER PCRs on ∆9 desaturase iGEM part but all were unsuccessful.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">We tried running several</span> USER PCRs on ∆9 desaturase iGEM part but all were unsuccessful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="intro">We received the ∆12</span> <del class="diffchange diffchange-inline">desaurase </del>(FAT-2) which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="intro">We received the ∆12</span> <ins class="diffchange diffchange-inline">desaturase </ins>(FAT-2) which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which was successful. Thus we cloned it into a PSB1C3 plasmid and <ins class="diffchange diffchange-inline">it </ins>can now be found in parts registry under </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>was successful. Thus we cloned it into a PSB1C3 plasmid and can now be found in parts registry under </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>SarahNielsenhttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=307484&oldid=prevSarahNielsen at 07:19, 17 October 20142014-10-17T07:19:50Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 07:19, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 5:</td>
<td colspan="2" class="diff-lineno">Line 5:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="intro">We wanted to clone</span> ∆9, ∆12 & ∆15 fatty acid desaturases into <del class="diffchange diffchange-inline">separately </del>plasmids by the use of USER</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="intro">We wanted to clone</span> ∆9, ∆12 & ∆15 fatty acid desaturases into <ins class="diffchange diffchange-inline">separate </ins>plasmids by the use of USER</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning. We wanted to use USER cloning to save some time and to ease the cloning process. Ahead of this </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning. We wanted to use USER cloning to save some time and to ease the cloning process. Ahead of this </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning, we needed the desaturases separately.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning, we needed the desaturases separately.<br><br></div></td></tr>
</table>SarahNielsenhttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=307183&oldid=prevSarahNielsen at 07:09, 17 October 20142014-10-17T07:09:53Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 07:09, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 13:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">We received the ∆12</span> desaurase (FAT-2) which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">We received the ∆12</span> desaurase (FAT-2) which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>was successful. Thus we cloned it into a PSB1C3 plasmid and can now be found in parts registry under </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>was successful. Thus we cloned it into a PSB1C3 plasmid and can now be found in parts registry under </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a><del class="diffchange diffchange-inline">(LINK)</del>. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">We tried to run</span> a colony PCR on the bacterium <i>Synechocystis sp.</i> PCC6803 in order for us to get the ∆15</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">We tried to run</span> a colony PCR on the bacterium <i>Synechocystis sp.</i> PCC6803 in order for us to get the ∆15</div></td></tr>
</table>SarahNielsenhttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=292071&oldid=prevUlrikasimone at 22:31, 16 October 20142014-10-16T22:31:47Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:31, 16 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 20:</td>
<td colspan="2" class="diff-lineno">Line 20:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">After several failed attempts</span> to run USER PCRs, we realized that we needed to prioritize and continue</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="intro">After several failed attempts</span> to run USER PCRs, we realized that we needed to prioritize and continue</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>without cloning ∆9, ∆12 & ∆15 into the separate plasmids, with the use of USER cloning.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>without cloning ∆9, ∆12 & ∆15 into the separate plasmids, with the use of USER cloning.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><br><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Ulrikasimonehttp://2014.igem.org/wiki/index.php?title=Team:SDU-Denmark/Tour43&diff=243810&oldid=prevSarahNielsen at 12:21, 15 October 20142014-10-15T12:21:43Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 12:21, 15 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 5:</td>
<td colspan="2" class="diff-lineno">Line 5:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We wanted to clone ∆9, ∆12 & ∆15 fatty acid desaturases into separately plasmids by the use of USER</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><span class="intro"></ins>We wanted to clone<ins class="diffchange diffchange-inline"></span> </ins>∆9, ∆12 & ∆15 fatty acid desaturases into separately plasmids by the use of USER</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning. We wanted to use USER cloning to save some time and to ease the cloning process. Ahead of this </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning. We wanted to use USER cloning to save some time and to ease the cloning process. Ahead of this </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning, we needed the desaturases separately.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cloning, we needed the desaturases separately.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We tried running several USER PCRs on ∆9 desaturase iGEM part but all were unsuccessful.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><span class="intro"></ins>We tried running several<ins class="diffchange diffchange-inline"></span> </ins>USER PCRs on ∆9 desaturase iGEM part but all were unsuccessful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We received the ∆12 desaurase (FAT-2) which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><span class="intro"></ins>We received the ∆12<ins class="diffchange diffchange-inline"></span> </ins>desaurase (FAT-2) which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>was successful. Thus we cloned it into a PSB1C3 plasmid and can now be found in parts registry under </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>was successful. Thus we cloned it into a PSB1C3 plasmid and can now be found in parts registry under </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>(LINK). Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>(LINK). Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We tried to run a colony PCR on the bacterium <i>Synechocystis sp.</i> PCC6803 in order for us to get the ∆15</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><span class="intro"></ins>We tried to run<ins class="diffchange diffchange-inline"></span> </ins>a colony PCR on the bacterium <i>Synechocystis sp.</i> PCC6803 in order for us to get the ∆15</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>desaturase. Unfortunately, this was unsuccessful.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>desaturase. Unfortunately, this was unsuccessful.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>After several failed attempts to run USER PCRs, we realized that we needed to prioritize and continue</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><span class="intro"></ins>After several failed attempts<ins class="diffchange diffchange-inline"></span> </ins>to run USER PCRs, we realized that we needed to prioritize and continue</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>without cloning ∆9, ∆12 & ∆15 into the separate plasmids, with the use of USER cloning.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>without cloning ∆9, ∆12 & ∆15 into the separate plasmids, with the use of USER cloning.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>SarahNielsen