Team:SDU-Denmark/Tour42

From 2014.igem.org

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Figure 1: Western blot showing E. coli wild-type and E. coli expressing OneProt at OD at 0.3, 0.8, 1.8 and an overnight  
Figure 1: Western blot showing E. coli wild-type and E. coli expressing OneProt at OD at 0.3, 0.8, 1.8 and an overnight  
culture. The membrane has been exposed for 10 minutes.
culture. The membrane has been exposed for 10 minutes.

Revision as of 07:36, 17 October 2014

OneProt

The pTet expression system and limonene synthase construct is evolved around one thing: the OneProt. We have made the pTet-OneProt construct in order for us to synthesize a nutritional protein with the correct ratio of essential amino acids and the correct ratio between essential and non-essential amino acids. The device is found as Bba_K1475000.

The protein is self-designed, so we wanted to test if the protein were expressed in E. coli K12 MG1655, by the use of Western blotting. The western blot was blottet with E. coli K12 MG1655 wild-type and E. coli expressing OneProt at different OD measures.

Figure 1: Western blot showing E. coli wild-type and E. coli expressing OneProt at OD at 0.3, 0.8, 1.8 and an overnight culture. The membrane has been exposed for 10 minutes.

The protein has a 3xFLAG tag and since bonds are showing, OneProt is expressed. However, from this western blot, we cannot see if the protein has been cut, just that it is expressed.