Team:SCAU-China

From 2014.igem.org

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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            <ul>
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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                <li class="on"><h3><a href="https://2014.igem.org/Team:SCAU-China">Home</a></h3></li>
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<br>
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                    <li>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SCAU-China&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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                    <h3><a href="https://2014.igem.org/Team:SCAU-China/Team">Team</a><i>+</i></h3>
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                        <a href="#">- Members</a>
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                            <a href="#">- Advisors</a>
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                    <h3><a href="https://2014.igem.org/Team:SCAU-China/Project">Project</a><i>+</i></h3>
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                        <a href="#">- Overview</a>
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                            <a href="#">- arcA knock out</a>
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                            <a href="#">- nadE overexpression</a>
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                            <a href="#">- ppsA promoter</a>
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<a href="https://2014.igem.org/Team:SCAU-China"style="color:#000000">Home </a> </td>
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                    <h3></h3>
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<a href="https://2014.igem.org/Team:SCAU-China/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=SCAU-China"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:SCAU-China/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:SCAU-China/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:SCAU-China/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:SCAU-China/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:SCAU-China/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:SCAU-China/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<!--requirements section -->
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<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
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<tr>
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<td width="45%"  valign="top">
 +
 
 +
<p> Please be sure to keep these links, your audience will want to find your: </p>
 +
 
 +
<!-- Links to other team pages -->
 +
<ul>
 +
<li><a href="https://2014.igem.org/Team:SCAU-China">Home</a> </li>
 +
<li><a href="https://2014.igem.org/Team:SCAU-China/Team">Team</a> </li>
 +
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=SCAU-China">Official Team Profile</a> </li>
 +
<li><a href="https://2014.igem.org/Team:SCAU-China/Project">Project</a> </li>
 +
<li><a href="https://2014.igem.org/Team:SCAU-China/Parts">Parts</a> </li>
 +
<li><a href="https://2014.igem.org/Team:SCAU-China/Modeling">Modeling</a> </li>
 +
<li><a href="https://2014.igem.org/Team:SCAU-China/Notebook">Notebook</a> </li>
 +
<li><a href="https://2014.igem.org/Team:SCAU-China/Safety">Safety</a> </li>
 +
<li><a href="https://2014.igem.org/Team:SCAU-China/Attributions">Attributions</a> </li>
 +
 
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</ul>
 +
 
 +
</td>
 +
 
 +
<td > </td>
 +
<td width="45%">
 +
 
 +
<p>There are a few wiki requirements teams must follow:</p>
 +
<ul>
 +
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload"> 2014.igem.org server</a>. </li>
 +
<li>All pages must be created under the team’s name space.</li>
 +
<li>As part of your documentation, keep the links from the menu to the left. </li>
 +
<li>Do not use flash in wiki code. </li>
 +
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
 +
</ul>
 +
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
 +
 
 +
</td>
 +
</tr>
 +
 
 +
 
 +
<tr> <td colspan="3"  height="15px"> </td></tr>
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
 +
 
 +
 
 +
<!--tips  -->
 +
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
 +
 
 +
<tr>
 +
<td width="45%" valign="top">
 +
<p>We are currently working on providing teams with some easy to use design templates.
 +
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
 +
 
 +
<ul>
 +
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 +
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 +
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 +
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 +
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 +
</ul>
 +
 
 +
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
 +
</td>
 +
 
 +
<td> </td>
 +
<td width="45%">  
 +
 
 +
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 +
 
 +
<ul>
 +
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 +
<li>Be clear about what you are doing and what you plan to do.</li>
 +
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 +
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 +
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 +
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
 +
<li>Have lots of fun! </li>
 +
 
 +
</td>
 +
</tr>
 +
 
 +
 
 +
 
 +
<tr> <td colspan="3"  height="15px"> </td></tr>
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
 +
 
 +
 
 +
<!--Project description  -->
 +
<tr><td colspan="3" > <h3> Project description  </h3></td></tr>
 +
 
 +
<tr>
 +
<td width="45%" valign="top">
 +
<p>For traditional energy resources are depleting rapidly in modern sociecty, new clean energy resources are highly desired to sustain the human development. In addition, fresh water shortage is a critical globle issue, which increases the demand of desalination of seawater despite its high-costed and energy-consuming processes. Microbial Fuel Cell (MFC) is a novel electricity generator, producing electric current via metabolization of waste organic substrates by electrogenic microorganisms. However, low output of power density limits the application of MFC. So in this project, we combined MFC with a seawater desalination device as a Microbial Desalination Cell (MDC), and tried to increase the power density by genetic modification of the microbes in three ways. Firstly, we knocked out the  arcA gene of E.coli, which encodes a negative regulatory transcriptional factor of enzymes in TCA Circle and reduces the metabolic rate under anaerobic condition, leading to increase the electricity output. Secondly, we over-expressed nadE gene to boot up the NAD+(H) level in the cells facilitating the electron transferring rate. Finaly, we incorporated porin OprF, a membrane channels for electron exchange, into the nadE-overexpressed microbes. Moreover, we optimized the desalination effeciency by improving the size of chamber, the shape of water path and the electrode composition in a traditional MDC device. In short, our project aims to enhence the electrogenic capacity with our genetically modified bacteria, so the sewage treatment and desalination plant can work together to achieve a prospective sustainable eco-device.

Revision as of 06:08, 14 October 2014


WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Requirements

Please be sure to keep these links, your audience will want to find your:

There are a few wiki requirements teams must follow:

  • All pages, images and files must be hosted on the 2014.igem.org server.
  • All pages must be created under the team’s name space.
  • As part of your documentation, keep the links from the menu to the left.
  • Do not use flash in wiki code.
  • The iGEM logo should be placed on the upper part of every page and should link to 2014.igem.org.

Visit the Wiki How To page for a complete list of requirements, tips and other useful information.

Tips

We are currently working on providing teams with some easy to use design templates.
In the meantime you can also view other team wikis for inspiration! Here are some very good examples

For a full wiki list, you can visit iGEM 2013 web sites and iGEM 2012 web sites lists.

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and what you plan to do.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2013 calendar
  • Have lots of fun!

Project description

For traditional energy resources are depleting rapidly in modern sociecty, new clean energy resources are highly desired to sustain the human development. In addition, fresh water shortage is a critical globle issue, which increases the demand of desalination of seawater despite its high-costed and energy-consuming processes. Microbial Fuel Cell (MFC) is a novel electricity generator, producing electric current via metabolization of waste organic substrates by electrogenic microorganisms. However, low output of power density limits the application of MFC. So in this project, we combined MFC with a seawater desalination device as a Microbial Desalination Cell (MDC), and tried to increase the power density by genetic modification of the microbes in three ways. Firstly, we knocked out the arcA gene of E.coli, which encodes a negative regulatory transcriptional factor of enzymes in TCA Circle and reduces the metabolic rate under anaerobic condition, leading to increase the electricity output. Secondly, we over-expressed nadE gene to boot up the NAD+(H) level in the cells facilitating the electron transferring rate. Finaly, we incorporated porin OprF, a membrane channels for electron exchange, into the nadE-overexpressed microbes. Moreover, we optimized the desalination effeciency by improving the size of chamber, the shape of water path and the electrode composition in a traditional MDC device. In short, our project aims to enhence the electrogenic capacity with our genetically modified bacteria, so the sewage treatment and desalination plant can work together to achieve a prospective sustainable eco-device.

Retrieved from "http://2014.igem.org/Team:SCAU-China"