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Team:Pitt
From 2014.igem.org
Pitt iGEM
Acne vulgaris is an extremely common skin disease, affecting more than 80% of teenagers across most cultures. Certain strains of the bacterium, Propionibacterium acnes, have been associated with acne, yet pathogenesis of acne remains unclear. Scientists are unable to study P. acnes because P. acnes is resistant to traditional genetic engineering techniques. Furthermore, the frequent use of antibiotics to treat acne is one of several factors leading to a dangerous rise in antibiotic-resistant bacteria.
We are developing a platform genetic vector to re-engineer P. acnes and to pioneer an alternative acne treatment using P. acnes as a skin probiotic. Specifically, we are designing a genetic tool, using a bacterial plasmid and transformation protocol, to study the connection between P. acnes and acne vulgaris through standard research tactics (e.g., gene knockouts). In addition, we are using our plasmid to introduce an anti-microbial peptide (cathelicidin) into the P. acnes genome, which could then be used as a probiotic to potentially treat acne without prescription antibiotics. We are developing other projects, alongside our cathelicidin-producing bacteria, to improve acne treatment and promote skin health. Specifically, we are investigating the pathogenesis of acne through two separate computational models. The first model is a bottom-up approach, using logic-based modeling to analyze the inflammation pathways in the skin leading to acne. The second model is a top-down approach, using linear regression techniques to examine trends in acne across different cultures. Lastly, our team has created a community outreach module, dubbed “Dermalicious,” to teach children about skin health by making an edible model of the skin.The diversity of our project stems directly from the diversity of our team, and we believe our multi-pronged approach is necessary when trying to understand and treat a complicated disease like acne.
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