http://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Protocols/Electro_elution_of_DNA_from_agarose_gel&feed=atom&action=historyTeam:Paris Saclay/Protocols/Electro elution of DNA from agarose gel - Revision history2024-03-29T12:19:39ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Protocols/Electro_elution_of_DNA_from_agarose_gel&diff=372188&oldid=prevMelaCriq: /* Electro elution of DNA from agarose gel */2014-10-18T00:02:22Z<p><span class="autocomment">Electro elution of DNA from agarose gel</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Electro elution of DNA from agarose gel=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Electro elution of DNA from agarose gel=</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Run samples on agarose gel as usual. When fragments are well separated, slice out bands of interest with a razor blade under the UV plate. Make slices as thin as possible (trim off excess agarose).Slices can be stored in microcentrifuge tubes in the refrigerator if necessary.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Fill the vat with TAE 0.5X buffer</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Close one of the wells of the saline bridge with a semi-permeable membrane, put the DNA in the large well</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Apply a 400V current after closing the vat, for 30min</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Reverse the polarity for 20s, pipet the solution from the small well and put it in a clean 1.5 ml tube</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Add 200µL of water and 600µL of phenol/chloroform/isoamyl alcohol ratio 25/24/1</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Vortex for 1 min</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Pipet the aqueous phase</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Precipitate the DNA with ethanol</ins></div></td></tr>
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</table>MelaCriqhttp://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Protocols/Electro_elution_of_DNA_from_agarose_gel&diff=84343&oldid=prevSC at 18:22, 22 August 20142014-08-22T18:22:04Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">=Electro elution of DNA from agarose gel=</ins></div></td></tr>
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