Protocols

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This page is under Maher's responsibility

• Deadline: 08/oct.
  • Uniformisation des textes.
  • Rappeler Fabio de creer la photo pour le protocol BioBrick Assembly
  • Changer les enzymes du protocol
  • Text explicatif des comment et pourquoi on a fait ces protocols
• Deadline: 12/oct
  • Final review.

1. Preparation of supercompetent E. coli cells
2. Transformation of CaCl2 supercompetent E. coli cells
3. Extraction of the Plasmid DNA from Bacteria by using NucleoSpin® Plasmid
4. Extraction of the Plasmid DNA from Bacteria without NucleoSpin® Plasmid
5. Gel Electrophoresis
6. Competent E. coli cells
7. PCR for the genomic DNA of bacteria
8. PCR clean-up
9. PCR for bacterial culture
10. Transformation of competent cells by electroporation
11. BioBrick Assembly
12. P1 Phages Stock
13. P1 phages transduction
14. Preparation and Transformation of competent E. coli by CaCl2
15. Cloning in pGEMTeasy
16. Electro elution of DNA from agarose gel