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Team:Paris Saclay/Project/Odor-free ecoli
From 2014.igem.org
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{{Team:Paris_Saclay/default_header}} | {{Team:Paris_Saclay/default_header}} | ||
| + | ==Countdown== | ||
| + | This page is under '''Romain''''s responsibility | ||
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| + | * Deadline: 08/oct. | ||
| + | ** Completed text. | ||
| + | * Deadline: 12/oct | ||
| + | ** Final review Philipe. | ||
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=Odor-free E.coli chassis= | =Odor-free E.coli chassis= | ||
==The E. coli Odor Free Chassis== | ==The E. coli Odor Free Chassis== | ||
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[[File:OdorFree.jpg|500px|center]] | [[File:OdorFree.jpg|500px|center]] | ||
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Revision as of 14:09, 4 October 2014
Countdown
This page is under Romain's responsibility
- Deadline: 08/oct.
- Completed text.
- Deadline: 12/oct
- Final review Philipe.
Odor-free E.coli chassis
The E. coli Odor Free Chassis
The E. coli odor free chassis=== Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene.
In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but this strain was too modified to be used for our project. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.
