Team:Paris Saclay/Project

From 2014.igem.org

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=Project=
=Project=
==Countdown==
==Countdown==
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* Deadline: 12/oct
* Deadline: 12/oct
** Final review par Maher.
** Final review par Maher.
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==Submenu==
 
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*[https://2014.igem.org/Team:Paris_Saclay/Project Introduction]
 
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*[https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations Inspirations]
 
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*[https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli Odor-free E.coli]
 
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*[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent Lemon Scent]
 
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*[https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System Salicylate Inducible System]
 
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*[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping Lemon Shaping]
 
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*[https://2014.igem.org/Team:Paris_Saclay/Project/Workshop Workshop]
 

Revision as of 20:13, 4 October 2014

Contents

Project

Countdown

This page is under Marie's responsibility

  • Deadline: 08/oct.
    • General introduction of the project.
    • Introduction text of each part of the project
  • Deadline: 12/oct
    • Final review par Maher.


Inspirations

A - The E. coli odor free chassis

Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene.

In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but this strain was too modified to be used for our project. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.

B - The Lemon Scent

The aim is to make three different populations of E.coli.

  1. The first is tranformed with a plasmid that contains the limonene synthase gene
  2. The second is transformed with a plasmid that contains the β-pinene synthase gene
  3. The third is tranformed with a plasmid that contains the geraniol synthase gene and the CAD gene to increase citral A and B production by the bacteria

We could thus play with the density of these populations for a better lemon scent, like cooking, putting the right quantity of each ingredient.

Don't worry more explanation will be there soon (melanie)

C - The Salicylate Inducible Suppressing System

D - The Lemon Shaping

The Workshop