Team:Paris Saclay/Notebook/September/8


Revision as of 15:41, 9 September 2014 by MelaCriq (Talk | contribs)


Lab Work

D-Lemon Scent

Checking PCR

Electrophoresis of the PCR made Friday


PCR with bacteria

From the petri dish (made here, I select some clone to do a PCR : clones 1-2-3 for cad clones 4-5 for PS clones 6 for LS

Cycle step Temperature Time Cycle

Bacteria lysis


5 min


Denaturation 94°C 30 s 25
Annealing 50°C 25 s 25
Extension 72°C 1 min 25
Final extension 72°C 10 min 1
Final extension 8°C hold 1