Team:Paris Saclay/Notebook/September/8
From 2014.igem.org
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(→PCR with bacteria) |
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clones 4-5 for PS | clones 4-5 for PS | ||
clones 6 for LS | clones 6 for LS | ||
+ | |||
+ | {| class="wikitable centre" width="50%" | ||
+ | |+ | ||
+ | |- | ||
+ | ! scope=col | component | ||
+ | ! scope=col | volume | ||
+ | |- | ||
+ | |H<sub>2</sub>O | ||
+ | |41.5μl | ||
+ | |- | ||
+ | |buffer | ||
+ | |5μl | ||
+ | |- | ||
+ | |dNTPs | ||
+ | |1μl | ||
+ | |- | ||
+ | |Primer 1 | ||
+ | |1μl | ||
+ | |- | ||
+ | |Primer 2 | ||
+ | |1μl | ||
+ | |- | ||
+ | |bacteria | ||
+ | |(about 1µl = 1 colony) | ||
+ | |- | ||
+ | |enzyme | ||
+ | |0.5μl | ||
+ | |} | ||
+ | |||
{| class="wikitable centre" width="80%" | {| class="wikitable centre" width="80%" |
Revision as of 15:42, 9 September 2014
Contents |
Lab Work
D-Lemon Scent
Checking PCR
Electrophoresis of the PCR made Friday
[photo]
PCR with bacteria
From the petri dish (made here, I select some clone to do a PCR : clones 1-2-3 for cad clones 4-5 for PS clones 6 for LS
component | volume |
---|---|
H2O | 41.5μl |
buffer | 5μl |
dNTPs | 1μl |
Primer 1 | 1μl |
Primer 2 | 1μl |
bacteria | (about 1µl = 1 colony) |
enzyme | 0.5μl |
Cycle step | Temperature | Time | Cycle |
---|---|---|---|
Bacteria lysis |
95°C |
5 min |
1 |
Denaturation | 94°C | 30 s | 25 |
Annealing | 50°C | 25 s | 25 |
Extension | 72°C | 1 min | 25 |
Final extension | 72°C | 10 min | 1 |
Final extension | 8°C | hold | 1 |