Team:Paris Saclay/Notebook/September/8

From 2014.igem.org

(Difference between revisions)
(PCR with bacteria)
(PCR with bacteria)
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clones 4-5 for PS
clones 4-5 for PS
clones 6 for LS
clones 6 for LS
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{| class="wikitable centre" width="50%"
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|+
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|-
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! scope=col | component
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! scope=col | volume
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|-
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|H<sub>2</sub>O
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|41.5μl
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|-
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|buffer
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|5μl
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|-
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|dNTPs
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|1μl
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|-
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|Primer 1
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|1μl
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|-
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|Primer 2
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|1μl
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|-
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|bacteria
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|(about 1µl = 1 colony)
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|-
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|enzyme
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|0.5μl
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|}
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{| class="wikitable centre" width="80%"
{| class="wikitable centre" width="80%"

Revision as of 15:42, 9 September 2014

Contents

Lab Work

D-Lemon Scent

Checking PCR

Electrophoresis of the PCR made Friday

[photo]

PCR with bacteria

From the petri dish (made here, I select some clone to do a PCR : clones 1-2-3 for cad clones 4-5 for PS clones 6 for LS

component volume
H2O 41.5μl
buffer 5μl
dNTPs 1μl
Primer 1 1μl
Primer 2 1μl
bacteria (about 1µl = 1 colony)
enzyme 0.5μl


Cycle step Temperature Time Cycle

Bacteria lysis

95°C

5 min

1

Denaturation 94°C 30 s 25
Annealing 50°C 25 s 25
Extension 72°C 1 min 25
Final extension 72°C 10 min 1
Final extension 8°C hold 1