Team:Paris Saclay/Notebook/September/8

From 2014.igem.org

(Difference between revisions)
(Checking PCR)
(PCR with bacteria)
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====PCR with bacteria====
====PCR with bacteria====
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From the petri dish ([http://2014.igem.org/Team:Paris_Saclay/Notebook/September/5#Transformation made here], I select some clone to do a PCR :
 +
clones 1-2-3  for cad
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clones 4-5 for PS
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clones 6 for LS
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 +
{| class="wikitable centre" width="80%"
 +
|+
 +
|-
 +
! scope=col | Cycle step
 +
! scope=col | Temperature
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! scope=col | Time
 +
! scope=col | Cycle
 +
|-
 +
| width="25%" |
 +
Initial denaturation
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| width="25%" |
 +
94°C
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| width="25%" |
 +
1 min
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| width="25%" |
 +
1
 +
|-
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| Denaturation
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| 94°C
 +
| 30 s
 +
| 25
 +
|-
 +
| Annealing
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| 50°C
 +
| 25 s
 +
| 25
 +
|-
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| Extension
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| 72°C
 +
| 1 min
 +
| 25
 +
|-
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| Final extension
 +
| 72°C
 +
| 10 min
 +
| 1
 +
|-
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| Final extension
 +
| 8°C
 +
| hold
 +
| 1
 +
|}

Revision as of 15:40, 9 September 2014

Contents

Lab Work

D-Lemon Scent

Checking PCR

Electrophoresis of the PCR made Friday

[photo]

PCR with bacteria

From the petri dish (made here, I select some clone to do a PCR : clones 1-2-3 for cad clones 4-5 for PS clones 6 for LS

Cycle step Temperature Time Cycle

Initial denaturation

94°C

1 min

1

Denaturation 94°C 30 s 25
Annealing 50°C 25 s 25
Extension 72°C 1 min 25
Final extension 72°C 10 min 1
Final extension 8°C hold 1