Team:Paris Saclay/Notebook/September/3

From 2014.igem.org

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(B- Construction of the fusion protein)
(PCR LS)
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====PCR LS====
====PCR LS====
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Electrophoresis of the PCR made [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/2#D-_Lemon_scent Yesterday]
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Electrophoresis of the PCR made [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/2#Limonene_synthase_PCR Yesterday]
[photo]
[photo]

Revision as of 15:45, 4 September 2014

Contents

LabWork

B- Construction of the fusion protein

We made a classic exttraction of plasmids from the liquid cultures.

Check by electrophoresis if it worked and send it for sequencing.


D- Lemon scent

by Mélanie

PCR LS

Electrophoresis of the PCR made Yesterday

[photo]

The PCR have success so I use the PCR clean up kit to purify it

[photo] Electrophoresis after purification

=Ligation

We already have some pPSI digested and dephosphorelated

So I do a ligation: