Team:Paris Saclay/Notebook/September/3

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(Created page with "==B- Construction of the fusion protein== We made a classic exttraction of plasmids from the liquid cultures. Check by electrophoresis if it worked and send it for sequencing.")
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==B- Construction of the fusion protein==
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==LabWork==
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===B- Construction of the fusion protein===
We made a classic exttraction of plasmids from the liquid cultures.
We made a classic exttraction of plasmids from the liquid cultures.
Check by electrophoresis if it worked and send it for sequencing.
Check by electrophoresis if it worked and send it for sequencing.
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 +
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===D- Lemon scent===
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''by Mélanie''
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====PCR LS====
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Electrophoresis of the PCR made [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/2#D-_Lemon_scent Yesterday]
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[photo]
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The PCR have success so I use the PCR clean up kit to purify it
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[photo] Electrophoresis after purification
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====Ligation===
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We already have some pPSI digested and dephosphorelated
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 +
So I do a ligation:

Revision as of 15:41, 4 September 2014

Contents

LabWork

B- Construction of the fusion protein

We made a classic exttraction of plasmids from the liquid cultures.

Check by electrophoresis if it worked and send it for sequencing.


D- Lemon scent

by Mélanie

PCR LS

Electrophoresis of the PCR made Yesterday

[photo]

The PCR have success so I use the PCR clean up kit to purify it

[photo] Electrophoresis after purification

=Ligation

We already have some pPSI digested and dephosphorelated

So I do a ligation:

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