Team:Paris Saclay/Notebook/September/2
From 2014.igem.org
Labwork
B-Contruction of the fusion protein
By Hv
We can see that the blue colonies of E.coli are not blue in a simple petri dish with ampicillin. It's a fail. So, to figure our problem we decided to do a sequencing of our plasmids.
We chose 6 colonies from an Xgal-IPTG petri dish:
- 1 completely blue
- 2 blue with a white ring around the blue
- 3 white colony
PS: we found that there is 2 types of blue colonies: completely blue and blue with a white ring around the blue)
We first did a liquid culture in 5 mL of LB.
D- Lemon scent
By Melanie
Limonene synthase Migration of the PCR done yesterday
photo
well 1= ladder
well 2-6 = pPS5
other well = LS PCR
As we see, there are nothing on our gel so we conclude that we failed to clone LS in pGMETeasy
pPPS5
the photo in the same as previously: We saw a band but we will check another time by PCR to be sure.
pPS3 and pPS4
Plasmid extraction using the plasmid DNA extraction kit and electrophoresis:
[photo]
We conclude that we have done a good manipulation.
Limonene synthase
As we saw that we don't have anything for LS, We try to do other PCR from the Biobrick (BBA762100). We use two enzyme: Dream taq and Vent taq