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Team:Paris Saclay/Notebook/September/16
From 2014.igem.org
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Test of the transformation made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 yesterday] to know if we had obtained a plasmid] | Test of the transformation made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 yesterday] to know if we had obtained a plasmid] | ||
| - | [[File:1609 PCR sur clones. | + | [[File:1609 PCR sur clones.jpg|400px]] |
primer to ckeck the direction of the insert | primer to ckeck the direction of the insert | ||
Revision as of 11:57, 26 September 2014
Contents |
Lab Work
By Mélanie
D- Lemon scent
Plasmids extraction
Extraction of the plasmid from the culture made yesterday(PS)
Digestion of PS topo plasmids
by PacI and PSTI
Ligation
After digestion, ligation overnight in PPS1
PCR Colony
Test of the transformation made yesterday to know if we had obtained a plasmid]
primer to ckeck the direction of the insert PPS2 : iPS72-96
PPS4 : ips72-97
= no insert
liquide culture
liquide culture from yesterday's colony to make stock tomorrow
PCR cad to cloning in pSBIC3
PCR and digestion PSTI/ecoRI and ligation
PCR GS
PCR to check if we have the insert in our topo plasmid
B- Construction of the fusion protein (color)
PCR
PCR to check if we have the insert in our topo plasmid
