Team:Paris Saclay/Notebook/September/16

From 2014.igem.org

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{{Team:Paris_Saclay/notebook_header}}
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=Tuesday 16th September=
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==Lab Work==
==Lab Work==
''By Mélanie''
''By Mélanie''
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===D- Lemon scent===
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===Lemon scent===
====Plasmids extraction====
====Plasmids extraction====
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by PacI and PSTI
by PacI and PSTI
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[[File:1609 digestion ps et gs par pacI.jpg|400px|center]]
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(well 2 and 3 : PacI digestion)
====Ligation====
====Ligation====
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Test of the transformation made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 yesterday] to know if we had obtained a plasmid]
Test of the transformation made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 yesterday] to know if we had obtained a plasmid]
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[photo]  
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[[File:1609 PCR sur clones.jpg|400px|center]]  
primer to ckeck the direction of the insert
primer to ckeck the direction of the insert
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PPS4 : ips72-97
PPS4 : ips72-97
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= no insert
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= no insert (just well 1-9)
====liquide culture====
====liquide culture====
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===B- Construction of the fusion protein (color)===
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===Construction of the fusion protein (color)===
====PCR====
====PCR====
PCR to check if we have the insert in our topo plasmid  
PCR to check if we have the insert in our topo plasmid  
[https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR see here]
[https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR see here]
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[[File:1609 chromo topo 2-9 jaune 10-17 bleu.jpg|400px|center]]
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well 2-9 = chromo yellow in topo plasmid
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well 10-17 = chromo blue in topo plasmid
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==Photo of the Day==
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[[File:Paris Saclay 2_september.jpg|300px|center]]
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Work of [http://ateliers-artistes-belleville.fr/artiste/therese-bichon/ Thérèse Bichon] , one of those few we have interviewed during this month of september.
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To learn more about the ethic aspect of our project, just click [https://2014.igem.org/Team:Paris_Saclay/Ethics here]
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 12:35, 14 October 2014

Contents

Tuesday 16th September

Lab Work

By Mélanie

Lemon scent

Plasmids extraction

Extraction of the plasmid from the culture made yesterday(PS)

Digestion of PS topo plasmids

by PacI and PSTI

1609 digestion ps et gs par pacI.jpg

(well 2 and 3 : PacI digestion)

Ligation

After digestion, ligation overnight in PPS1


PCR Colony

Test of the transformation made yesterday to know if we had obtained a plasmid]

1609 PCR sur clones.jpg

primer to ckeck the direction of the insert PPS2 : iPS72-96

PPS4 : ips72-97

= no insert (just well 1-9)

liquide culture

liquide culture from yesterday's colony to make stock tomorrow

PCR cad to cloning in pSBIC3

PCR and digestion PSTI/ecoRI and ligation

PCR GS

PCR to check if we have the insert in our topo plasmid

see here


Construction of the fusion protein (color)

PCR

PCR to check if we have the insert in our topo plasmid

see here

1609 chromo topo 2-9 jaune 10-17 bleu.jpg

well 2-9 = chromo yellow in topo plasmid well 10-17 = chromo blue in topo plasmid

Photo of the Day

Paris Saclay 2 september.jpg

Work of Thérèse Bichon , one of those few we have interviewed during this month of september.

To learn more about the ethic aspect of our project, just click here

Back to the calendar