Team:Paris Saclay/Notebook/September/16

From 2014.igem.org

(Difference between revisions)
(PCR Colony)
(PCR Colony)
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PPS4 : ips72-97
PPS4 : ips72-97
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= no insert
+
= no insert (just well 1-9)
====liquide culture====
====liquide culture====

Revision as of 12:00, 26 September 2014

Contents

Lab Work

By Mélanie

D- Lemon scent

Plasmids extraction

Extraction of the plasmid from the culture made yesterday(PS)

Digestion of PS topo plasmids

by PacI and PSTI

Ligation

After digestion, ligation overnight in PPS1


PCR Colony

Test of the transformation made yesterday to know if we had obtained a plasmid]

1609 PCR sur clones.jpg

primer to ckeck the direction of the insert PPS2 : iPS72-96

PPS4 : ips72-97

= no insert (just well 1-9)

liquide culture

liquide culture from yesterday's colony to make stock tomorrow

PCR cad to cloning in pSBIC3

PCR and digestion PSTI/ecoRI and ligation

PCR GS

PCR to check if we have the insert in our topo plasmid

see here


B- Construction of the fusion protein (color)

PCR

PCR to check if we have the insert in our topo plasmid

see here