Team:Paris Saclay/Notebook/September/16
From 2014.igem.org
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''By Mélanie'' | ''By Mélanie'' | ||
===D- Lemon scent=== | ===D- Lemon scent=== | ||
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+ | ====Plasmids extraction==== | ||
+ | Extraction of the plasmid from the culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#liquide_culture_of_some_PS_clone_.28topo.29 yesterday](PS) | ||
+ | |||
+ | ====Digestion of PS topo plasmids==== | ||
+ | |||
+ | by PacI and PSTI | ||
+ | |||
+ | ====Ligation==== | ||
+ | |||
+ | After digestion, ligation overnight in PPS1 | ||
+ | |||
+ | |||
+ | ====PCR Colony==== | ||
+ | Test of the transformation made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 yesterday] to know if we had obtained a plasmid] | ||
+ | |||
+ | [photo] | ||
+ | |||
+ | primer to ckeck the direction of the insert | ||
+ | PPS2 : iPS72-96 | ||
+ | PPS4 : ips72-97 | ||
+ | |||
+ | ====liquide culture==== | ||
+ | liquide culture from [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_of_LS_GS_CAS_.28topo.29_to_do_stock yesterday's colony] | ||
+ | to make stock tomorrow | ||
+ | |||
+ | ===PCR cad to cloning in pSBIC3=== | ||
+ | PCR and digestion PSTI/ecoRI and ligation | ||
+ | |||
+ | ====PCR GS==== | ||
+ | PCR to check if we have the insert in our topo plasmid | ||
+ | |||
+ | [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR_pSCBC3_cloning see here] | ||
+ | |||
+ | |||
+ | ===B- Construction of the fusion protein (color)=== | ||
+ | ====PCR=== | ||
+ | PCR to check if we have the insert in our topo plasmid | ||
+ | |||
+ | [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR see here] |
Revision as of 16:19, 17 September 2014
Contents |
Lab Work
By Mélanie
D- Lemon scent
Plasmids extraction
Extraction of the plasmid from the culture made yesterday(PS)
Digestion of PS topo plasmids
by PacI and PSTI
Ligation
After digestion, ligation overnight in PPS1
PCR Colony
Test of the transformation made yesterday to know if we had obtained a plasmid]
[photo]
primer to ckeck the direction of the insert PPS2 : iPS72-96 PPS4 : ips72-97
liquide culture
liquide culture from yesterday's colony to make stock tomorrow
PCR cad to cloning in pSBIC3
PCR and digestion PSTI/ecoRI and ligation
PCR GS
PCR to check if we have the insert in our topo plasmid
B- Construction of the fusion protein (color)
=PCR
PCR to check if we have the insert in our topo plasmid