Team:Paris Saclay/Notebook/September/16

From 2014.igem.org

(Difference between revisions)
(Lab Work)
(D- Lemon scent)
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''By Mélanie''
''By Mélanie''
===D- Lemon scent===
===D- Lemon scent===
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====Plasmids extraction====
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Extraction of the plasmid from the culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#liquide_culture_of_some_PS_clone_.28topo.29 yesterday](PS)
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====Digestion of PS topo plasmids====
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by PacI and PSTI
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====Ligation====
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After digestion, ligation overnight in PPS1
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====PCR Colony====
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Test of the transformation made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 yesterday] to know if we had obtained a plasmid]
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[photo]
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primer to ckeck the direction of the insert
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PPS2 :  iPS72-96
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PPS4 : ips72-97
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====liquide culture====
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liquide culture from [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_of_LS_GS_CAS_.28topo.29_to_do_stock yesterday's colony]
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to make stock tomorrow
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===PCR cad to cloning in pSBIC3===
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PCR and digestion PSTI/ecoRI and ligation
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====PCR GS====
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PCR to check if we have the insert in our topo plasmid
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[https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR_pSCBC3_cloning see here]
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===B- Construction of the fusion protein (color)===
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====PCR===
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PCR to check if we have the insert in our topo plasmid
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[https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR see here]

Revision as of 16:19, 17 September 2014

Contents

Lab Work

By Mélanie

D- Lemon scent

Plasmids extraction

Extraction of the plasmid from the culture made yesterday(PS)

Digestion of PS topo plasmids

by PacI and PSTI

Ligation

After digestion, ligation overnight in PPS1


PCR Colony

Test of the transformation made yesterday to know if we had obtained a plasmid]

[photo]

primer to ckeck the direction of the insert PPS2 : iPS72-96 PPS4 : ips72-97

liquide culture

liquide culture from yesterday's colony to make stock tomorrow

PCR cad to cloning in pSBIC3

PCR and digestion PSTI/ecoRI and ligation

PCR GS

PCR to check if we have the insert in our topo plasmid

see here


B- Construction of the fusion protein (color)

=PCR

PCR to check if we have the insert in our topo plasmid

see here