Team:Paris Saclay/Notebook/September/15

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{{Team:Paris_Saclay/notebook_header}}
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=Monday 15th September=
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==Lab Work==
==Lab Work==
''by Mélanie''
''by Mélanie''
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===D-lemon scent===
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===lemon scent===
====Transformation with the ligation (futur pPS2/4)====
====Transformation with the ligation (futur pPS2/4)====
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PCR of GS : primer with ecoRI and PSTI restriction site
PCR of GS : primer with ecoRI and PSTI restriction site
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===B- Construction of the fusion protein (color)===
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===Construction of the fusion protein (color)===
====PCR====
====PCR====
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====transformation for stock of the w6 clone====
====transformation for stock of the w6 clone====
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==Photo of the Day==
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[[File:Paris Saclay 15_september.jpg|600px|center]]
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 13:13, 14 October 2014

Contents

Monday 15th September

Lab Work

by Mélanie

lemon scent

Transformation with the ligation (futur pPS2/4)

liquide culture of some PS clone (topo)

Transformation of LS GS CAS (topo) to do stock

PCR pSCBC3 cloning

PCR of GS : primer with ecoRI and PSTI restriction site

Construction of the fusion protein (color)

PCR

We have decided to separate the two protein So I do a PCR to have the yellow and the blue protein and i cloned them in a topo plasmid.

transformation for stock of the w6 clone

Photo of the Day

Paris Saclay 15 september.jpg

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