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From 2014.igem.org

Contents 1 Lab Work 1.1 D-Lemon Scent 1.1.1 Plasmid extraction 1.1.2 digestion 1.1.3 Purification 1.1.4 culture

Lab Work

by Mélanie

D-Lemon Scent

Plasmid extraction

From the liquide culture made Yesterday I do a plasmid extraction.

[photo]

these clone had sent to sequencing

digestion

I digest with PacI the plasmid that contain LS and GS

component	volume
H2O	5μl
buffer	3μl
PacI	1μl
Plasmid	20μl

[Photo]

Purification

Electrophoresis to isolate the insert

culture

As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B