Team:Paris Saclay/Notebook/September/10

From 2014.igem.org

(Difference between revisions)
(digestion)
(Plasmid extraction)
Line 7: Line 7:
From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/9#PCR Yesterday] I do a plasmid extraction.
From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/9#PCR Yesterday] I do a plasmid extraction.
-
[[File:1009 vérif extraction plasmides.jpg|200px]]
+
[[File:1009 vérif extraction plasmides.jpg|300px]]
these clones (LS GS CAD) had sent to sequencing
these clones (LS GS CAD) had sent to sequencing

Revision as of 13:28, 14 September 2014

Contents

Lab Work

by Mélanie

D-Lemon Scent

Plasmid extraction

From the liquide culture made Yesterday I do a plasmid extraction.

1009 vérif extraction plasmides.jpg

these clones (LS GS CAD) had sent to sequencing problem with ps

digestion

I digest with PacI the plasmid that contain LS and GS

component volume
H2O 5μl
buffer 3μl
PacI 1μl
Plasmid 20μl

200PX

Purification

Electrophoresis to isolate the insert and purification with a kit = I failed

culture

As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B


Modeling

by Arnaud, Eugène, Juliette and Pierre

Meeting about modeling with advisor Claire to talk about the achievement of our model

  • Diffusion of O2 in the gel : maths is OK but biologist need more drawings.
  • Bacterial growth on the surface : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab.
  • Translecture system : maybe we have to give up this part of our work because of the new project.
  • Diffusion of the limonene : Eugène has focused on the concentration of limonene in the atmosphere.