"
Team:Paris Saclay/Notebook/September/10
From 2014.igem.org
(Difference between revisions)
MelaCriq (Talk | contribs)
(Created page with "==Lab Work== ''by Mélanie'' ===D-Lemon Scent=== ====Plasmid extraction==== From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/...")
Newer edit →
(Created page with "==Lab Work== ''by Mélanie'' ===D-Lemon Scent=== ====Plasmid extraction==== From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/...")
Newer edit →
Revision as of 15:24, 10 September 2014
Contents |
Lab Work
by Mélanie
D-Lemon Scent
Plasmid extraction
From the liquide culture made Yesterday I do a plasmid extraction.
[photo]
these clone had sent to sequencing
digestion
I digest with PacI the plasmid that contain LS and GS
| component | volume |
|---|---|
| H2O | 5μl |
| buffer | 3μl |
| PacI | 1μl |
| Plasmid | 20μl |
[Photo]
Purification
Electrophoresis to isolate the insert
culture
As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B
