Team:Paris Saclay/Notebook/September/10

From 2014.igem.org

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=Wednesday 10th September=
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==Lab Work==
==Lab Work==
''by Mélanie''
''by Mélanie''
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====culture====
====culture====
As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B
As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B
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==Photo of the Day==
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[[File:Paris Saclay 2_september.jpg|600px|center]]
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[http://emmanuelhirsch.fr/ Emmanuel Hirsch] , one of those few we have interviewed during this month of september.
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To learn more about the ethic aspect of our project, just click [https://2014.igem.org/Team:Paris_Saclay/Ethics here]
==Modeling==
==Modeling==

Revision as of 06:34, 14 October 2014

Contents

Wednesday 10th September

Lab Work

by Mélanie

D-Lemon Scent

Plasmid extraction

From the liquide culture made Yesterday I do a plasmid extraction.

1009 vérif extraction plasmides.jpg

these clones (LS GS CAD) had sent to sequencing problem with ps

digestion

I digest with PacI the plasmid that contain LS and GS

component volume
H2O 5μl
buffer 3μl
PacI 1μl
Plasmid 20μl

200PX

Purification

Electrophoresis to isolate the insert and purification with a kit = I failed

culture

As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B


Photo of the Day

Paris Saclay 2 september.jpg

Emmanuel Hirsch , one of those few we have interviewed during this month of september.

To learn more about the ethic aspect of our project, just click here

Modeling

by Arnaud, Eugène, Juliette and Pierre

Meeting about modeling with advisor Claire to talk about the achievement of our model

  • Diffusion of O2 in the gel : maths is OK but biologist need more drawings.
  • Bacterial growth on the surface : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab.
  • Translecture system : maybe we have to give up this part of our work because of the new project.
  • Diffusion of the limonene : Eugène has focused on the concentration of limonene in the atmosphere.

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