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Team:Paris Saclay/Notebook/September/10
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From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/9#PCR Yesterday] I do a plasmid extraction. | From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/9#PCR Yesterday] I do a plasmid extraction. | ||
| - | [ | + | [[File:1009 vérif extraction plasmides.jpg|200px]] |
these clones (LS GS CAD) had sent to sequencing | these clones (LS GS CAD) had sent to sequencing | ||
| + | problem with ps | ||
====digestion==== | ====digestion==== | ||
Revision as of 13:28, 14 September 2014
Contents |
Lab Work
by Mélanie
D-Lemon Scent
Plasmid extraction
From the liquide culture made Yesterday I do a plasmid extraction.
these clones (LS GS CAD) had sent to sequencing problem with ps
digestion
I digest with PacI the plasmid that contain LS and GS
| component | volume |
|---|---|
| H2O | 5μl |
| buffer | 3μl |
| PacI | 1μl |
| Plasmid | 20μl |
Purification
Electrophoresis to isolate the insert and purification with a kit = I failed
culture
As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B
Modeling
by Arnaud, Eugène, Juliette and Pierre
Meeting about modeling with advisor Claire to talk about the achievement of our model
- Diffusion of O2 in the gel : maths is OK but biologist need more drawings.
- Bacterial growth on the surface : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab.
- Translecture system : maybe we have to give up this part of our work because of the new project.
- Diffusion of the limonene : Eugène has focused on the concentration of limonene in the atmosphere.
