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Team:Paris Saclay/Notebook/September/10
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As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B | As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B | ||
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| + | ==Modeling== | ||
| + | |||
| + | ''by Arnaud, Eugène, Juliette and Pierre'' | ||
| + | |||
| + | Meeting about modeling with advisor Claire to talk about the achievement of our model | ||
| + | * ''Diffusion of O<sub>2</sub> in the gel'' : maths is OK but biologist need more drawings. | ||
| + | * ''Bacterial growth on the surface'' : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab. | ||
| + | * ''Translecture system'' : maybe we have to give up this part of our work because of the new project. | ||
| + | * ''Diffusion of the limonene'' : Eugène has focused on the concentration of limonene in the atmosphere. | ||
Revision as of 11:00, 11 September 2014
Contents |
Lab Work
by Mélanie
D-Lemon Scent
Plasmid extraction
From the liquide culture made Yesterday I do a plasmid extraction.
[photo]
these clone had sent to sequencing
digestion
I digest with PacI the plasmid that contain LS and GS
| component | volume |
|---|---|
| H2O | 5μl |
| buffer | 3μl |
| PacI | 1μl |
| Plasmid | 20μl |
[Photo]
Purification
Electrophoresis to isolate the insert
culture
As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B
Modeling
by Arnaud, Eugène, Juliette and Pierre
Meeting about modeling with advisor Claire to talk about the achievement of our model
- Diffusion of O2 in the gel : maths is OK but biologist need more drawings.
- Bacterial growth on the surface : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab.
- Translecture system : maybe we have to give up this part of our work because of the new project.
- Diffusion of the limonene : Eugène has focused on the concentration of limonene in the atmosphere.
