Team:Paris Saclay/Notebook/September/1

From 2014.igem.org

(Difference between revisions)
(B-Construction of the fusion protein)
(D-Lemon scent)
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'' by melanie''
'' by melanie''
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Limone synthase
+
====Limone synthase====
We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26#Streaking_of_colonies_transformed_by_BBa_K762100.2BpGEMTeasy_.28LS.29 26 August]We do PCR of all the clones:
We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26#Streaking_of_colonies_transformed_by_BBa_K762100.2BpGEMTeasy_.28LS.29 26 August]We do PCR of all the clones:
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PPS5
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====PPS5====
We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase)
We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase)
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PPS3 and PPS4
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====PPS3 and PPS4====
We need to work on this plasmid tomorrow so we do some culture from the stock
We need to work on this plasmid tomorrow so we do some culture from the stock

Revision as of 15:42, 4 September 2014

Contents

Lab Work

B-Construction of the fusion protein

'by Hoang Vu'

We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent prick out.

D-Lemon scent

by melanie

Limone synthase

We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from 26 AugustWe do PCR of all the clones:

We use the Protocol - PCR for bacterial culture and we use the same PCR condition than on the August 8 but with the appropriate Primer and Biobrick.


PPS5

We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) So we do a PCR in the same condition than August 20


PPS3 and PPS4

We need to work on this plasmid tomorrow so we do some culture from the stock