Team:Paris Saclay/Notebook/July/8

From 2014.igem.org

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(Plasmid DNA preparation:)
(Transformation in DH5α of the following biobricks:)
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol]
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NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.
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NB : The protocol was modified. Instead of spreading 10<sup>-1</sup> and 10<sup>-2</sup> solutions of bacteria we took a non diluted solution and a 10<sup>-1</sup> solution.
====Plasmid DNA preparation:====
====Plasmid DNA preparation:====

Revision as of 14:15, 26 August 2014

Contents

Tuesday 8th July

Lab work

Rehydration of the following biobricks:

by Sean

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)

Protocol:

  1. Pierce foil cover of well containing biobrick of interest.
  2. Add 10 μl of water in well.
  3. Transfer contents of well into a 1.5 ml microcentrifuge tube.

Transformation in DH5α of the following biobricks:

by Mathieu & Maher

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)
  • Control + : pUC18 (20ng)
  • Control -

Protocol

NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.

Plasmid DNA preparation:

by Romain & Sean

Plasmids used:

  • pJBEI-6409

E. coli strain used:

  • XL1-Blue

Protocol

Members present:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

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