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Team:Paris Saclay/Notebook/July/3
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In 2ml Eppendorf : | In 2ml Eppendorf : | ||
| - | + | :4 eppendorf MG1655 (0.1ml + 50µl CaCl) | |
| - | + | :4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl) | |
Add 100µl of the different phages. | Add 100µl of the different phages. | ||
'''Wait 20 minutes!''' | '''Wait 20 minutes!''' | ||
| - | + | Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M | |
'''Wait 1 or 2 hours at 37°C with agitation''' | '''Wait 1 or 2 hours at 37°C with agitation''' | ||
Revision as of 14:09, 11 July 2014
Thursday 3rd July
Lab Work
1 - Phages P1 transduction in MG1655Z1
Mathieu
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation
Pour in LB Kan petri dishes, incubate at 37°C.(Turn the petri dishes over when there is no more liquid in)
