Team:Paris Saclay/Notebook/July/16

From 2014.igem.org

(Difference between revisions)
(Lab work)
(Wednesday 16th July)
Line 3: Line 3:
==Lab work==
==Lab work==
-
=== 1 - Transformation in DH5α ===
+
===1 - Transformation in DH5α===
''by Fabio, Maher and Romain''
''by Fabio, Maher and Romain''
Line 30: Line 30:
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
 +
 +
===2 - Plasmid DNA Purification===
 +
 +
''by Mathieu''
 +
 +
*J23100 (x2)
 +
*J23106 (x2)
 +
*J23114 (x2)
 +
 +
from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/15 15th July]
 +
 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
==Reunion==
==Reunion==

Revision as of 15:49, 21 July 2014

Contents

Wednesday 16th July

Lab work

1 - Transformation in DH5α

by Fabio, Maher and Romain

We transformed in DH5α the following Biobrick:

  • BBa J23119

Samples:

  1. Concentrated
  2. Undiluted
  3. Diluted 10^-1
  4. Control

Results:

  1. Concentrated: 99 colonies
  2. Undiluted: 5 colonies
  3. Diluted: 0
  4. Control: 0

Conclusion:

Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.

Protocol

2 - Plasmid DNA Purification

by Mathieu

  • J23100 (x2)
  • J23106 (x2)
  • J23114 (x2)

from Bacterial Culture made the 15th July

Protocol

Reunion

Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)

Reunion: Lab meeting to discuss the project. (afternoon)

Meeting with Evry's Team. (night) Arnaud represents us

Back to the calendar