Team:Paris Saclay/Notebook/August/22

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We've made a electrophoresis to chech the digestion.
We've made a electrophoresis to chech the digestion.
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==== Ligation of LS/GS/PS in pPSI====
==== Ligation of LS/GS/PS in pPSI====

Revision as of 10:12, 22 August 2014

Contents

Friday 22nd August

Lab work

D - Lemon Scent

pPSI digestion

40µl pPSI

2µl H2O

2µL PacI

5µL FAST digest buffer

pPSI dephosphorylation

After 2 hours, add 1.5µL of Alkaline Phosphatase (AP) and let it 1 hour.

Then, inactivation of AP at 65°C during 15 minutes.

We've made a electrophoresis to chech the digestion.

(photo)

Ligation of LS/GS/PS in pPSI

For each synthase,put:

  1. 10 µL of synthase
  2. 2 µL of pPSI
  3. 2 µL of buffer
  4. 5 µL of water
  5. 1 µL of ligase

And let it in the freezer during 3 hours.


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