Team:Paris Saclay/Notebook/August/20

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(Difference between revisions)
(Bacterial culture)
(Bacterial culture)
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''by Melanie''
''by Melanie''
 +
Preculture 20ml of DH5a pPSI (apra 1/2000)
Preculture 20ml of DH5a pPSI (apra 1/2000)

Revision as of 13:44, 20 August 2014

Wednesday 20th August

Back to the calendar

D- Lemon scent

Bacterial culture

by Laetitia

Here are the results of the bacterial culture of yesterday

20 08 bacterial culture.jpg

Bacteria with the pGEMTeasy without the DNA insert are blue and the bacteria with the pGEMTeasy and the DNA insert (PS, GS or LS gene) are white

Then, we have chosen 3 white colonies for each DNA insert and we have launched 3 corresponding liquid cultures :

- 5mL LB + ampiciline (1/1000e)

- 1 white colony

The 9 cultures are incubated at 37°C


by Melanie

Preculture 20ml of DH5a pPSI (apra 1/2000)