Team:Paris Saclay/Notebook/August/14

From 2014.igem.org

(Difference between revisions)
(Transformation of DH5α)
(D - Lemon Scent)
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''by Melanie''
''by Melanie''
-
From the 20 ml of odor free culture (pJBEI Apra) done yesterday (''by Laetitia'')
+
From the 20 ml of odor free culture (pJBEI Apra - pPSI) done yesterday (''by Laetitia'')
Plasmid extraction without Nucleospin
Plasmid extraction without Nucleospin
 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue protocol]
=== Transformation of DH5α ===
=== Transformation of DH5α ===

Revision as of 11:02, 20 August 2014

Contents

Thursday 14th August

Lab Work

by Melanie

Preparation of DH5a CaCl2 Competent cell

Protocol

D - Lemon Scent

by Melanie

From the 20 ml of odor free culture (pJBEI Apra - pPSI) done yesterday (by Laetitia)

Plasmid extraction without Nucleospin protocol

Transformation of DH5α

by Sean & Eugene

We prepare a LB agar environment with a concentration of:

  • 0.5 mM of IPTG
  • 0.008% of Xgal
  • 1/2000 of Ampicilline

and we spread our preparation in a petri dish and incubate overnight at 37°C.


Protocol

Human Practices

Art & Design

by Terry

We found an interesting colorless medium named M63.

Preparation of M63 medium, concentration 5X, one liter basis :

  • 10g (NH4)2 SO4
  • 68g KH2 PO4
  • 2.5mg FeSO4 7H2O
  • Adjust to pH 7 with KOH

The concentrated medium is diluted to 1X with sterile water and the following solution ( one liter basis ) :

  • 1 ml 1M MgSO4 7H2O
  • 10 ml 20% carbon source (sugar or glycerol)
  • 0.1 ml 0.5% vitamin B1

100 ml of M63 has been prepared for future manipulations.


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