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Team:Paris Saclay/Notebook/August/14
From 2014.igem.org
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''by Melanie'' | ''by Melanie'' | ||
| - | From the 20 ml of odor free culture (pJBEI Apra - pPSI) done yesterday (''by Laetitia'') | + | From the 20 ml of odor free culture (pJBEI Apra - pPSI) done yesterday (''by Laetitia'') |
Plasmid extraction without Nucleospin | Plasmid extraction without Nucleospin | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue protocol] | ||
| - | + | + Stock (DH5a pJBEI + apra) | |
=== Transformation of DH5α === | === Transformation of DH5α === | ||
Revision as of 10:41, 10 September 2014
Contents |
Thursday 14th August
Lab Work
by Melanie
Preparation of DH5a CaCl2 Competent cell
D - Lemon Scent
by Melanie
From the 20 ml of odor free culture (pJBEI Apra - pPSI) done yesterday (by Laetitia)
Plasmid extraction without Nucleospin protocol
+ Stock (DH5a pJBEI + apra)
Transformation of DH5α
by Sean & Eugene
We prepare a LB agar environment with a concentration of:
- 0.5 mM of IPTG
- 0.008% of Xgal
- 1/2000 of Ampicilline
and we spread our preparation in a petri dish and incubate overnight at 37°C.
Human Practices
Art & Design
by Terry
We found an interesting colorless medium named M63.
Preparation of M63 medium, concentration 5X, one liter basis :
- 10g (NH4)2 SO4
- 68g KH2 PO4
- 2.5mg FeSO4 7H2O
- Adjust to pH 7 with KOH
The concentrated medium is diluted to 1X with sterile water and the following solution ( one liter basis ) :
- 1 ml 1M MgSO4 7H2O
- 10 ml 20% carbon source (sugar or glycerol)
- 0.1 ml 0.5% vitamin B1
100 ml of M63 has been prepared for future manipulations.
