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Team:Paris Saclay/Notebook/August/13
From 2014.igem.org
Contents |
Wednesday 13th August
Lab work
D - Lemon scent
culture of pJBEI6409 bacteria
by Mélanie culture in LB of some clones obtain yesterday
Gel electrophoresis of BBa_K762100
by Sean
Cloning BBa_K517003 and p cola in pGEMTeasy
by Eugene and Sean
| components | volumes |
|---|---|
| PCR purified p cola/ BBa_K517003 | 7 μl |
| DreamTaq buffer 10X | 1 μl |
| dATP | 2 µl |
| DreamTaq | 1 µl |
incubate for 30 minutes at 70°C
| components | volumes |
|---|---|
| 2xT4ligase buffer | 10 μl |
| pGEMTeasy | 1 μl |
| DNA insert | 4.7 µl at 16.8 ng/µl |
| 2T4DNA ligase | 1 µl |
| H2O | 3.3 µl |
| components | volumes |
|---|---|
| 2xT4ligase buffer | 10 μl |
| pGEMTeasy | 1 μl |
| DNA insert | 5.6 µl at 16.8 ng/µl |
| 2T4DNA ligase | 1 µl |
| H2O | 2.4 µl |
Culture of transformed bacteria
We used 3 colony of bacteria transformed with PJBEI+Apra (11/08) to lauch 3 cultures
-two cultures in 5mL LB+Apra (1/2000)
-one culture in 20mL LB+Apra (1/2000)
37°C - 24h
by Laetitia
Human Practices
Art & Design
by Terry
Unmolding yesterday's preparation. The mold was not hermetically closed, the chromoprotein is expressed in presence of oxygen.
It looks like blue tasty space cake but...
...when unmolded, you can only see a little ring of blue at 100% ( non-diluted preculture ).In fact, bacteria didn't grow.
I forgot that there are no nutrients in agar alone, bacteria won't grow without appropriate medium. I can't use LB because of its color. I must find a colorless medium.
