Team:Paris Saclay/Labwork

From 2014.igem.org

Revision as of 09:28, 14 October 2014 by MelaCriq (Talk | contribs)

Contents

Labwork

Planning

A compléter: We started the work in June, with the research project scope and bibliography. In early July we began the phase of experiments, which was completed in the first half of September. We students organized ourselves into four groups of summer internship. The groups were formed by virtually all members of the team, so even students from areas other than biology could cooperate with the project in the laboratory experiments. Into four working groups, the laboratory experiments. To ensure the smooth development of the project by all students, the work had a life cycle.   a cycle of "aprendisagem" >> "Training" >> 'Development' >> "Transmission of acquired knowledge."

A - The E. coli odor-free chassis

  • Culture of MG1655 and MG1655Z1 strains- 30th June
  • P1 phage stock preparation for the transduction of the Delta-tnaA::Kan - 2nd July
  • P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - 3rd July
  • Cultures of MG1655 and MG1655Z1 transductants - 4th July
  • Transformations test of competent cells MG1655 and MG1655Z1 - 10th July
  • Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - 11th July
  • Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - 17th July
  • Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with plasmid BT340- 18th July
  • Results of the transformation - 21st July
  • Preparation and transformation of competent MG1655 and MG1655Z1 transductants - 22nd July
  • PCR verification of the strains grown - 25th July
  • Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - 28th July
  • PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - 29th July
  • Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with plasmid BT340 - 30th July
  • Clone isolation by streak of Delta-tnaA MG1655 and MG1655Z1 on LB dishes - 1st August
  • Clone isolation by streak of Delta-tnaA MG1655 and MG1655Z1 on LB and kan dishes - 4th August
  • PCR verification of the final strains Delta-tnaA MG1655 and MG1655Z1 - 5th August
  • Final stock of MG1655 Delta-tnaA - 6th August

B - The Lemon Scent

  • Rehydration of BioBricks BBa_J45014, BBa_K517003 - here
  • Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - here
  • Extraction of p cola plasmid DNA - here
  • PCR targeting with DY330 and pJBEI-6409 - here
  • Extraction and electrophoresis of BBa_K762100 with pSB1C3 - here
  • Gel electrophoresis of p cola - here
  • Transformation of DY330 with pJBEI-6409 - here
  • Liquid culture of DY330 - here
  • Transformation of DY330 with pJBEI-6409 - 29th here
  • Culture of DY330 + pJBEI-6409 - here
  • Transformation of DY330 with pJBEI-6409 - here
  • Electroporation of DY330+pJBEI-6409 - here

C - Salicylate Inducible Suppressing System

D - The Lemon Shaping

Countdown

This page is under Fabio's responsibility

  • Deadline: 08/oct.
    • Planning of each subproject
  • Deadline: 12/oct
    • Final review Solenne