Team:Paris Bettencourt/Results

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<div class=project id=project1>
<div class=project id=project1>
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<p class=text2></br></br></br></br></p><p class=text2><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell"><h6 class=right>Teen Spirit</h6></a></p>
+
<p class=text2></br></br></br></br></p><p class=text1><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell"><span class=hauteur>Teen Spirit</span></a></p>
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<p class=text2>-Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source. Determined the critical smell detection limit of 2-nonenal.</br>
+
-
-Analyzed 2-nonenal tolerance and degradation in natural isolates.</br></p>
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-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Odor_Library">Smell The Roses</a></h6><br>
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<p class=text1>
<p class=text1>
-Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.</br>
-Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.</br>
-We produced the smells that compose the main odor categories perceived by humans.</br>
-We produced the smells that compose the main odor categories perceived by humans.</br>
-BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019 </br></p>
-BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019 </br></p>
 +
 +
<p class=text1>
 +
<span class=hauteur><a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Odor_Library">Smell The Roses</a></span></br>-Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source. -Determined the critical smell detection limit of 2-nonenal.</br>
 +
-Analyzed 2-nonenal tolerance and degradation in natural isolates.</br></p>
</div>
</div>
<p>
<p>
<div class=project id=project2>
<div class=project id=project2>
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<p class=text2></p>
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<p class=text1></p>
</div>
</div>
<div class=project id=project3>
<div class=project id=project3>
</br></br>
</br></br>
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<h6 class=right><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Foot_Odor">Goody Two Shoes</a></h6><br>
+
<p class=text1>
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<p class=text2>-Demonstrated that mutant strains of <i>B. subtilis</i> produce less odor.</br>
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Foot_Odor">Goody Two Shoes</a></span><br>-Demonstrated that mutant strains of <i>B. subtilis</i> produce less odor.</br>
-Measured growth and viability of mutants in synthetic sweat media and on socks.</br> </p>
-Measured growth and viability of mutants in synthetic sweat media and on socks.</br> </p>
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/TMAU">Something Fishy</a></h6><br>
 
<p class=text1>
<p class=text1>
 +
<span class=hauteur><a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/TMAU">Something Fishy</a></span></br>
-Cloned <i>tmm</i> into <i>E.coli</i> using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.</br>
-Cloned <i>tmm</i> into <i>E.coli</i> using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.</br>
-Characterized and quantified the activity of TMM by a colorimetric assay.</br>
-Characterized and quantified the activity of TMM by a colorimetric assay.</br>
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  </p>
  </p>
</div>
</div>
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<div class=project id=project4>
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<div class=project id=project4>
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<p class=text2></p>
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-
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<p class=text1></p>
<p class=text1></p>
</div>
</div>
<div class=project id=project5>
<div class=project id=project5>
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<p class=text2></p>
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<p class=text1></p>
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<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Eliminate_Smell">Don't Sweat It</a></h6><br>
+
 
<p class=text1>
<p class=text1>
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Expressed the odor gene <i>agaA</i> in <i>E. coli</i> (BBa_K1403002).</li>
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Eliminate_Smell">Don't Sweat It</a></span><br>
-
Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.</li>
+
-Expressed the odor gene <i>agaA</i> in <i>E. coli</i> (BBa_K1403002).</br>
-
Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.</li>
+
-Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.</br>
-
Formulated a simple skin cream encorporating live bacteria.</li></p>
+
-Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.</br>
 +
-Formulated a simple skin cream encorporating live bacteria.</br></p>
</div>
</div>
<div class=project id=project6>
<div class=project id=project6>
<div class=project id=project7>
<div class=project id=project7>
-
<h6 class=right><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab study</a></h6><br>
+
<p class=text1>
-
<p class=text2> Successfully built the three devices</li>
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab study</a></span><br> -Successfully built the three devices</br>
-
Sequenced and gel verified the three devices</li>
+
-Sequenced and gel verified the three devices</li>
-
Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader</li>Reported the results on the <a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab Study</a> page of the iGEM wiki</li>
+
-Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader</br>-Reported the results on the <a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab Study</a> page of the iGEM wiki</br>
-
Completed the description of the <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> (Device 1)  
+
-Completed the description of the <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> (Device 1) </br>
-
Submitted and send parts: <a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> (Device 2) and <a href="http://parts.igem.org/Part:BBa_K1403001">BBa_K1403001</a> (Device 3) to the Biobricks registry</li> </p>
+
-Submitted and send parts: <a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> (Device 2) and <a href="http://parts.igem.org/Part:BBa_K1403001">BBa_K1403001</a> (Device 3) to the Biobricks registry</br> </p>
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</div>
</div>
<div class=project id=project8>
<div class=project id=project8>
-
<h6 class=right><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Collaboration">Collaborations</a></h6><br>
+
 
-
<p class=text2><b>The Smell Game</b>
+
<p class=text1>
 +
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Collaboration">Collaborations</a></span><br><b>The Smell Game</b>
The web platform continues to evolve, mostly thanks to the people who give their samples and play the game. At this time, users can give a sample of his own sweat, play with the samples of others and then obtain a position of their smell.The SmellGame project relies on a web platform available here :  
The web platform continues to evolve, mostly thanks to the people who give their samples and play the game. At this time, users can give a sample of his own sweat, play with the samples of others and then obtain a position of their smell.The SmellGame project relies on a web platform available here :  
http://smellofus.synbio4all.org.
http://smellofus.synbio4all.org.
Join us and play! </p>
Join us and play! </p>
-
+
 
-
+
<p class=text1>
-
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Human_Practice">Human practice</a></span><br><b>MOOC</b></br>
-
<p class=text2></p>
+
-Created a MOOC with 21 lessons to help high school students build a team.</br>
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Human_Practice">Human practice</a></h6><br>
+
-More than 50 people annotated text in Genius and 15 teachers will use the tool next year.</br>
-
<p class=text1><b>MOOC</b></br>
+
-20 organizations and iGEM teams around the world have agreed to promote it.</br>
-
Created a MOOC with 21 lessons to help high school students build a team.
+
-A student club was created in CRI (Paris) to continue the support for MOOC iGEM HS.</br></br>
-
More than 50 people annotated text in Genius and 15 teachers will use the tool next year.
+
-
20 organizations and iGEM teams around the world have agreed to promote it.
+
-
A student club was created in CRI (Paris) to continue the support for MOOC iGEM HS.</br></br>
+
<b>Citizen science</b></br>
<b>Citizen science</b></br>
-
More than 600 citizen scientists participated.
+
-More than 600 citizen scientists participated.</br>
-
Developing a Smell game for sample analysis.
+
-Developing a Smell game for sample analysis</br>
-
Living lab event in Cite de Sciences et de l'Industrie.
+
-Living lab event in Cite de Sciences et de l'Industrie.</br>
-
Watching a scary movie had little to no affect on body odor intensity
+
-Watching a scary movie had little to no affect on body odor intensity.</br>
</br></br>
</br></br>

Latest revision as of 03:57, 18 October 2014

Smell
the roses
Teen
spirit
Something
fishy
Goody
two shoes
Don't
sweat it





Teen Spirit

-Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.
-We produced the smells that compose the main odor categories perceived by humans.
-BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019

Smell The Roses
-Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source. -Determined the critical smell detection limit of 2-nonenal.
-Analyzed 2-nonenal tolerance and degradation in natural isolates.



Goody Two Shoes
-Demonstrated that mutant strains of B. subtilis produce less odor.
-Measured growth and viability of mutants in synthetic sweat media and on socks.

Something Fishy
-Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
-Characterized and quantified the activity of TMM by a colorimetric assay.
-Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).

Don't Sweat It
-Expressed the odor gene agaA in E. coli (BBa_K1403002).
-Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.
-Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.
-Formulated a simple skin cream encorporating live bacteria.

Interlab study
-Successfully built the three devices
-Sequenced and gel verified the three devices -Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
-Reported the results on the Interlab Study page of the iGEM wiki
-Completed the description of the BBa_I20260 (Device 1)
-Submitted and send parts: BBa_K1403000 (Device 2) and BBa_K1403001 (Device 3) to the Biobricks registry

Collaborations
The Smell Game The web platform continues to evolve, mostly thanks to the people who give their samples and play the game. At this time, users can give a sample of his own sweat, play with the samples of others and then obtain a position of their smell.The SmellGame project relies on a web platform available here : http://smellofus.synbio4all.org. Join us and play!

Human practice
MOOC
-Created a MOOC with 21 lessons to help high school students build a team.
-More than 50 people annotated text in Genius and 15 teachers will use the tool next year.
-20 organizations and iGEM teams around the world have agreed to promote it.
-A student club was created in CRI (Paris) to continue the support for MOOC iGEM HS.

Citizen science
-More than 600 citizen scientists participated.
-Developing a Smell game for sample analysis
-Living lab event in Cite de Sciences et de l'Industrie.
-Watching a scary movie had little to no affect on body odor intensity.


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