Team:Paris Bettencourt/Project/Foot Odor

From 2014.igem.org

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<h6>Aims and Achievement</h6><br>
<h6>Aims and Achievement</h6><br>
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<p class=text1>Foot odor is generally perceived as socially awkward and negative. There are a many commercially available solutions for this problem. </br></br>However, all current products indiscriminately target all bacteria of the microbiome, which can have negative effects on skin health. In this project we aim to develop a targeted approach to prevent foot odor, without destroying the beneficial microbes.</p>
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<p class=text1>Foot odor is generally perceived as socially awkward and negative. Although there are a many commercially available solutions for this problem, current products indiscriminately target bacteria on the foot skin microbiome. These type of products can have negative effects on skin health and microbiome dynamics. We aim to develop a targeted approach to prevent foot odor, by selectively killing microbes responsible for the biosynthesis of volatile compounds which compose the characteristic <i>stinky</i> feet smell, without destroying the beneficial microbes.</p>
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<p class=text2><img id=image2 src="https://static.igem.org/mediawiki/2014/b/b1/Footfigure1PB.png"></p>
<h6>Introduction</h6><br>
<h6>Introduction</h6><br>
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<p class=text1>Foot odor (or bromhidrosis) is a unique type of body odor that is generally considered to be unpleasant.</br> </br>The malodorous compounds are produced by bacteria metabolizing sweat, which is almost odorless, into volatile, unpleasant compounds. </br>Bacillus subtilis, a common bacterium that lives on the foot is thought to produce the malodorous molecules behind this socially awkward condition. One of those molecules made by B. subtilis uses the leucine degradation pathway to produce isovaleric acid, which smells like parmesan cheese, which is considered to be to dominant smell of bromhidrosis.</p>
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<p class=text1>Foot odor (or bromhidrosis) is a condition in which a particular type of body odor, generally considered to be unpleasant, gets produced by bacteria during the catabolism of nutrients present in the sweat. </br> </br>Although sweat is almost odorless, the microbial volatile compounds that are formed as by-products of bacterial metabolism are perceived as an unpleasant smell. </br> <i>Bacillus subtilis<i/>, a common bacterium that lives on feet and it is thought to produce the malodorous molecules behind this socially awkward condition. One of the molecules produced by <i>B. subtilis<i/> uses the leucine degradation pathway to produce isovaleric acid, which smells like parmesan cheese. It is considered to be to dominant odor tone of bromhidrosis.</p>
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<p class=text2></br>Fig1</p>
<p class=text2></br>Fig1</p>
<h6>Methods</h6><br>
<h6>Methods</h6><br>
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<p class=text1>We hypothesize that bad foot odor could be eradicated by perturbing the Leucine degradation pathway.</br>
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<p class=text1>We hypothesized that bad foot odor could be eradicated by perturbing the leucine degradation pathway. We chose leucine dehydrogenase and iso-valeryl coA to perturb the leucine degradation pathway. Our goal here is to have two different strategies to inhibit isovaleric acid production. our first strategy focus on producing B.subtilis which will not be capable of producing leucine, so that it has to utilize all the leucine it could get from the sweat for its own survival rather than the production of isovaleric acid. Our second strategy focus on developing B.subtilis strain, which will be capable of producing leucine but not isovaleric acid.</p>
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We choose leucine dehydrogenase and iso-valeryl coA to perturb the leucine degradation pathway. Our goal here is to have two different strategies to inhibit isovaleric acid production. our first strategy focus on producing B.subtilis which will not be capable of producing leucine, so that it has to utilize all the leucine it could get from the sweat for its own survival rather than the production of isovaleric acid. Our second strategy focus on developing B.subtilis strain, which will be capable of producing leucine but not isovaleric acid.</p>
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Revision as of 16:20, 15 October 2014

BACKGROUND

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AIMS

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RESULTS

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Aims and Achievement Introduction Results Methods References

Aims and Achievement

Foot odor is generally perceived as socially awkward and negative. Although there are a many commercially available solutions for this problem, current products indiscriminately target bacteria on the foot skin microbiome. These type of products can have negative effects on skin health and microbiome dynamics. We aim to develop a targeted approach to prevent foot odor, by selectively killing microbes responsible for the biosynthesis of volatile compounds which compose the characteristic stinky feet smell, without destroying the beneficial microbes.

Introduction

Foot odor (or bromhidrosis) is a condition in which a particular type of body odor, generally considered to be unpleasant, gets produced by bacteria during the catabolism of nutrients present in the sweat.

Although sweat is almost odorless, the microbial volatile compounds that are formed as by-products of bacterial metabolism are perceived as an unpleasant smell.
Bacillus subtilis, a common bacterium that lives on feet and it is thought to produce the malodorous molecules behind this socially awkward condition. One of the molecules produced by B. subtilis uses the leucine degradation pathway to produce isovaleric acid, which smells like parmesan cheese. It is considered to be to dominant odor tone of bromhidrosis.


Fig2

Fig3

Results

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo.


Fig1

Methods

We hypothesized that bad foot odor could be eradicated by perturbing the leucine degradation pathway. We chose leucine dehydrogenase and iso-valeryl coA to perturb the leucine degradation pathway. Our goal here is to have two different strategies to inhibit isovaleric acid production. our first strategy focus on producing B.subtilis which will not be capable of producing leucine, so that it has to utilize all the leucine it could get from the sweat for its own survival rather than the production of isovaleric acid. Our second strategy focus on developing B.subtilis strain, which will be capable of producing leucine but not isovaleric acid.

References

- ref1
- ref2

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
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