http://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&feed=atom&action=historyTeam:Oxford/safety - Revision history2024-03-29T05:56:35ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=375222&oldid=prevPhillo92 at 00:28, 18 October 20142014-10-18T00:28:15Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacteria are contained within <a href="https://2014.igem.org/Team:Oxford/biopolymer_containment#show2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacteria are contained within <a href="https://2014.igem.org/Team:Oxford/biopolymer_containment#show2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>agarose beads</u></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>agarose beads</u></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the concentration rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Instead of this happening, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the concentration rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container <ins class="diffchange diffchange-inline">(pore size: 0.22 micrometres) </ins>in order to prevent them from being disposed down the sink. Instead of this happening, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</div></td></tr>
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</table>Phillo92http://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=374021&oldid=prevCorinnaO at 00:17, 18 October 20142014-10-18T00:17:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Introduction</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Introduction</h1></div></td></tr>
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</table>CorinnaOhttp://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=373974&oldid=prevCorinnaO: proofread2014-10-18T00:17:22Z<p>proofread</p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacteria are contained within <a href="https://2014.igem.org/Team:Oxford/biopolymer_containment#show2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacteria are contained within <a href="https://2014.igem.org/Team:Oxford/biopolymer_containment#show2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>agarose beads</u></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>agarose beads</u></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the <del class="diffchange diffchange-inline">concentrate </del>rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Instead, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the <ins class="diffchange diffchange-inline">concentration </ins>rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Instead <ins class="diffchange diffchange-inline">of this happening</ins>, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</div></td></tr>
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</table>CorinnaOhttp://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=360030&oldid=prevFrancescadonnellan at 22:08, 17 October 20142014-10-17T22:08:45Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Policy and Practices element of our project has asked how iGEM projects can grow from small lab studies into a commercial/industrial/medical product used by many people. An essential part of this research has been thinking about the safety of synthetic biology and specifically of the bioremediation technology we have developed, were it to be in widespread use. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Policy and Practices element of our project has asked how iGEM projects can grow from small lab studies into a commercial/industrial/medical product used by many people. An essential part of this research has been thinking about the safety of synthetic biology and specifically of the bioremediation technology we have developed, were it to be in widespread use. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The new risks associated with the growth of our project are broadly similar to those related to synthetic biology generally: the possibility of spread of antibiotic resistance from synthetic organisms into the natural environment, and the possible escape of synthetic organisms into the natural environment. Specific risks associated with <del class="diffchange diffchange-inline">out </del>project include the risk to lab workers from working with chlorinated solvents (although this is relatively easy to manage). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The new risks associated with the growth of our project are broadly similar to those related to synthetic biology generally: the possibility of spread of antibiotic resistance from synthetic organisms into the natural environment, and the possible escape of synthetic organisms into the natural environment. Specific risks associated with <ins class="diffchange diffchange-inline">our </ins>project include the risk to lab workers from working with chlorinated solvents (although this is relatively easy to manage). </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>There may be potential for the abuse of the <del class="diffchange diffchange-inline">BioBeads </del>being developed by our team as this technology will allow bacteria to survive in hostile environments. However, we consider that the potential benefits from the application of our design far outweigh any possible risks. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>There may be potential for the abuse of the <ins class="diffchange diffchange-inline">agarose bacteria-containing beads </ins>being developed by our team as this technology will allow bacteria to survive in hostile environments. However, we consider that the potential benefits from the application of our design far outweigh any possible risks. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacteria are contained within agarose beads that will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 mM. The bacteria will die when the concentration rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Our product will include instructions explaining to users how the beads can be disposed of safely (for example by sterilising with boiling water before disposal).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacteria are contained within agarose beads that will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 mM. The bacteria will die when the concentration rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Our product will include instructions explaining to users how the beads can be disposed of safely (for example by sterilising with boiling water before disposal).</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Risks to the safety and health of the general public (if any biological materials escaped from your lab) and risks to the environment:</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Risks to the safety and health of the general public (if any biological materials escaped from your lab) and risks to the environment:</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We are not working with any pathogenic microorganisms or sequences that could introduce <del class="diffchange diffchange-inline">it</del>. The vectors we are using are designed for prokaryotes exclusively and none of the sequences expressed are under the control of mammalian promoters in order to avoid their expression in human cells.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We are not working with any pathogenic microorganisms or sequences that could introduce <ins class="diffchange diffchange-inline">pathogenicity</ins>. The vectors we are using are designed for prokaryotes exclusively and none of the sequences expressed are under the control of mammalian promoters in order to avoid their expression in human cells.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The <del class="diffchange diffchange-inline">strains are disabled and do not have a </del>risk <del class="diffchange diffchange-inline">of harming </del>animals <del class="diffchange diffchange-inline">or </del>plants<del class="diffchange diffchange-inline">. Similarly, the vectors and sequences do not pose a risk for those as well</del>. The GMOs do not carry any additional risks for wild-type (unmodified) organisms.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The <ins class="diffchange diffchange-inline">above is also true regarding the </ins>risk <ins class="diffchange diffchange-inline">to other </ins>animals <ins class="diffchange diffchange-inline">and </ins>plants. The GMOs do not carry any additional risks for wild-type (unmodified) organisms.</div></td></tr>
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</table>Francescadonnellanhttp://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=358781&oldid=prevPhillo92 at 21:56, 17 October 20142014-10-17T21:56:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Features we have designed to minimise any risks:</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Features we have designed to minimise any risks:</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our bacteria are contained within <del class="diffchange diffchange-inline">agarose beads </del><a href="https://2014.igem.org/Team:Oxford/biopolymer_containment#show2"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our bacteria are contained within <a href="https://2014.igem.org/Team:Oxford/biopolymer_containment#show2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>agarose beads</u></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>agarose beads</u></a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the concentrate rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Instead, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the concentrate rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Instead, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</div></td></tr>
</table>Phillo92http://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=358752&oldid=prevPhillo92 at 21:55, 17 October 20142014-10-17T21:55:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Features we have designed to minimise any risks:</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Features we have designed to minimise any risks:</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our bacteria are contained within agarose beads which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the concentrate rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Instead, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our bacteria are contained within agarose beads <ins class="diffchange diffchange-inline"> <a href="https://2014.igem.org/Team:Oxford/biopolymer_containment#show2"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><u>agarose beads</u></a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the concentrate rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Instead, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
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</table>Phillo92http://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=358372&oldid=prevPhillo92 at 21:51, 17 October 20142014-10-17T21:51:24Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our work will not introduce or increase any pathogenicity or virulence of any of the microorganisms we are working with. The safety system of the Department ensures that only members of the Department have access to its facilities and that the microorganisms and genetic parts we are using will not leave the laboratory by any means. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our work will not introduce or increase any pathogenicity or virulence of any of the microorganisms we are working with. The safety system of the Department ensures that only members of the Department have access to its facilities and that the microorganisms and genetic parts we are using will not leave the laboratory by any means. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We decided to work with microorganisms that belong to risk group 1 exclusively. We will be wearing gloves, safety glasses and lab coats throughout our entire lab work and will carry out sterile techniques in order to avoid any contamination. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We decided to work with microorganisms that belong to risk group 1 exclusively. We will be wearing gloves, safety glasses and lab coats throughout our entire lab work and will carry out sterile techniques in order to avoid any contamination. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We have arranged safe containment of the microorganisms we are working with so that these cannot leave the laboratory. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We have arranged safe containment of the microorganisms we are working with so that these cannot leave the laboratory.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><h1>Features we have designed to minimise any risks:</h1></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Our bacteria are contained within agarose beads which will control the concentration of DCM which they are exposed to. Eventually these agarose beads will begin to degrade naturally over time, exposing the bacteria to a higher DCM concentration of up to 200 millimolar. The bacteria will die when the concentrate rises above the level they can tolerate, thus we have a kill switch inherently integrated into our design. In addition, the agarose beads will be trapped by a filter within the DCMation container in order to prevent them from being disposed down the sink. Instead, our product will include instructions explaining to users how the beads can be disposed of safely (for example by using virkon).</ins></div></td></tr>
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</table>Phillo92http://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=357732&oldid=prevPhillo92 at 21:44, 17 October 20142014-10-17T21:44:28Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Risks to security through malicious mis-use by individuals, groups, or countries and measures we are taking to reduce this risk:</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Risks to security through malicious mis-use by individuals, groups, or countries and measures we are taking to reduce this risk:</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">ur </del>work will not introduce or increase any pathogenicity or virulence of any of the microorganisms we are working with. The safety system of the Department ensures that only members of the Department have access to its facilities and that the microorganisms and genetic parts we are using will not leave the laboratory by any means. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Our </ins>work will not introduce or increase any pathogenicity or virulence of any of the microorganisms we are working with. The safety system of the Department ensures that only members of the Department have access to its facilities and that the microorganisms and genetic parts we are using will not leave the laboratory by any means. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We decided to work with microorganisms that belong to risk group 1 exclusively. We will be wearing gloves, safety glasses and lab coats throughout our entire lab work and will carry out sterile techniques in order to avoid any contamination. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We decided to work with microorganisms that belong to risk group 1 exclusively. We will be wearing gloves, safety glasses and lab coats throughout our entire lab work and will carry out sterile techniques in order to avoid any contamination. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We have arranged safe containment of the microorganisms we are working with so that these cannot leave the laboratory. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We have arranged safe containment of the microorganisms we are working with so that these cannot leave the laboratory. </div></td></tr>
</table>Phillo92http://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=357701&oldid=prevPhillo92 at 21:44, 17 October 20142014-10-17T21:44:11Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Risks to the safety and health of team members, or other people working in the lab:</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Risks to the safety and health of team members, or other people working in the lab:</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The E.coli, DM4 and Pseudomonas strains used do not present any risk to humans (ACDP hazard group 1), as indicated in the table above, and none of the sequences expressed are under the control of mammalian promoters in order to avoid their expression in human cells. Good microbiological practice including the wearing of gloves, labcoats and eye protection will be followed at all times.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The E.coli, DM4 and Pseudomonas strains used do not present any risk to humans (ACDP hazard group 1), as indicated in the table above, and none of the sequences expressed are under the control of mammalian promoters in order to avoid their expression in human cells. Good microbiological practice including the wearing of gloves, labcoats and eye protection will be followed at all times.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>Phillo92http://2014.igem.org/wiki/index.php?title=Team:Oxford/safety&diff=357677&oldid=prevPhillo92 at 21:43, 17 October 20142014-10-17T21:43:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are not working with any pathogenic microorganisms or sequences that could introduce it. The vectors we are using are designed for prokaryotes exclusively and none of the sequences expressed are under the control of mammalian promoters in order to avoid their expression in human cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are not working with any pathogenic microorganisms or sequences that could introduce it. The vectors we are using are designed for prokaryotes exclusively and none of the sequences expressed are under the control of mammalian promoters in order to avoid their expression in human cells.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The strains are disabled and do not have a risk of harming animals or plants. Similarly, the vectors and sequences do not pose a risk for those as well. The GMOs do not carry any additional risks for wild-type (unmodified) organisms.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The strains are disabled and do not have a risk of harming animals or plants. Similarly, the vectors and sequences do not pose a risk for those as well. The GMOs do not carry any additional risks for wild-type (unmodified) organisms.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h1>Risks to security through malicious mis-use by individuals, groups, or countries and measures we are taking to reduce this risk:</h1></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">ur work will not introduce or increase any pathogenicity or virulence of any of the microorganisms we are working with. The safety system of the Department ensures that only members of the Department have access to its facilities and that the microorganisms and genetic parts we are using will not leave the laboratory by any means. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We decided to work with microorganisms that belong to risk group 1 exclusively. We will be wearing gloves, safety glasses and lab coats throughout our entire lab work and will carry out sterile techniques in order to avoid any contamination. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We have arranged safe containment of the microorganisms we are working with so that these cannot leave the laboratory. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
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</table>Phillo92