Team:Oxford/protocols/Reviving Freeze-Dried Cultures
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Latest revision as of 12:39, 24 July 2014
Reviving Freeze-Dried Cultures
- Remove the freeze-dried strain from the cold room.
- In the lamina flow hood, cut the top of the seal of the ampoule off with scissors.
- Pipette 0.5 mL of the appropriate growth medium into the ampoule and mix by pipetting up and down, being careful to avoid frothing.
- Leave on the bench for 40 minutes to resuspend.
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