Team:Oxford/modellingA

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[DCM] calibration for solution-vapour equilibration

Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a binary solution/ideal gas, based on literature solubility data, confirming negligible [DCM] deviation:



p(DCM) /kPa

n(g) /mol

V(DCM) added /ml

n(DCM)(l) unadjusted /mol

[DCM] unadjusted /M

n(DCM)(l) adjusted /mol

[DCM] adjusted /M

[DCM] % deviation

(30°C, 5mM)

1.56E-04

3.94E-12

6.4E-03

1.0E-04

2.0E-02

1.0E-04

2.0E-02

3.9E-06

(30°C, 10mM)

3.12E-04

7.88E-12

3.2E-03

5.0E-05

1.0E-02

5.0E-05

1.0E-02

1.6E-05

(30°C, 20mM)

6.24E-04

1.58E-11

1.6E-03

2.5E-05

5.0E-03

2.5E-05

5.0E-03

6.3E-05










(37°C, 5mM)

1.56E-04

3.94E-12

6.4E-03

1.0E-04

2.0E-02

1.0E-04

2.0E-02

3.9E-06

(37°C, 10mM)

3.12E-04

7.88E-12

3.2E-03

5.0E-05

1.0E-02

5.0E-05

1.0E-02

1.6E-05

(37°C, 20mM)

6.24E-04

1.58E-11

1.6E-03

2.5E-05

5.0E-03

2.5E-05

5.0E-03

6.3E-05