Team:Oxford/biosensor
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The promoter of dcmA (DCM dehalogenase gene) is placed upstream of sfGFP; therefore we will get a fluorescent output instead of dcmA expression as in the native bacterium. Repression or activation of the dcmA promoter relies on the regulatory protein DcmR which responds to the [DCM]; in our genetic circuit, the dcmR gene is constitutively expressed.<br><br> | The promoter of dcmA (DCM dehalogenase gene) is placed upstream of sfGFP; therefore we will get a fluorescent output instead of dcmA expression as in the native bacterium. Repression or activation of the dcmA promoter relies on the regulatory protein DcmR which responds to the [DCM]; in our genetic circuit, the dcmR gene is constitutively expressed.<br><br> | ||
- | Click on the | + | Click on the characterisation link below to find out more about the genetic regulatory network we are characterising and engineering to produce our biosensor!<br><br> |
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By having an electronic circuit we can quickly adapt our DCMation kit to give the same user-friendly output (a green LED comes on on top of the bench-top kit to indicate the contents can be poured away) depending on the result of our characterisation of the action of DcmR. This meant that we could develop both the genetic circuit and the physical realisation of our product at the same time rather than sequentially - saving us time!<br><br> | By having an electronic circuit we can quickly adapt our DCMation kit to give the same user-friendly output (a green LED comes on on top of the bench-top kit to indicate the contents can be poured away) depending on the result of our characterisation of the action of DcmR. This meant that we could develop both the genetic circuit and the physical realisation of our product at the same time rather than sequentially - saving us time!<br><br> | ||
- | Click on the | + | Click on the realisation link below to see more about our fluorescence detecting circuit!<br><br> |
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Revision as of 18:16, 6 October 2014
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