Team:Oxford/bioremediation

From 2014.igem.org

(Difference between revisions)
 
(15 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Oxford/templates/header}}
 +
<html>
<html>
-
<head>
 
-
  <style type="text/css">
 
-
  @import url(http://fonts.googleapis.com/css?family=PT+Serif|Open+Sans:300italic,400,300);
 
-
  html, body {
+
<style>
-
    max-width: 100%;
+
h1blue2 {
-
    overflow-x: hidden;
+
font-size: 130%;
-
  }
+
-
 
+
-
  body {
+
-
    background-color: #EBEBEB;
+
-
    margin: 0px;
+
-
    padding: 0;
+
-
    border: 0;
+
-
    vertical-align: baseline;
+
-
    font-family: 'Open Sans', 'helvetica', sans-serif;
+
-
    font-size: 10px;
+
-
    font-weight: 300;
+
-
  }
+
-
 
+
-
  #content {
+
-
    border:none;
+
-
    width:100%;
+
-
    margin:0;
+
-
    padding:0;
+
-
    background:none;
+
-
  }
+
-
 
+
-
  #catlinks {
+
-
    display:none;
+
-
  }
+
-
 
+
-
  #contentSub {
+
-
    display:none;
+
-
  }
+
-
 
+
-
  #menubar {
+
-
    width: auto;
+
-
    margin-top:0.5%;
+
-
}
+
-
 
+
-
  .left-menu:hover {
+
-
    background-color: transparent;
+
-
  }
+
-
  #menubar li a {
+
-
    background-color: transparent;
+
-
  }
+
-
  #menubar:hover {
+
-
    color: gray;
+
-
  }
+
-
  #menubar li a {
+
-
    color: #B5B5B5;
+
-
  }
+
-
  #menubar:hover li a {
+
-
    color: gray;
+
-
  }
+
-
 
+
-
  #menubar.left-menu.noprint ul li:last-child {
+
-
  display:none;
+
-
  }
+
-
 
+
-
  #search-controls {
+
-
    display:none;
+
-
  }
+
-
 
+
-
  #top-section {
+
-
    height:0px;
+
-
    width:100%;
+
-
    border:none;
+
-
  }
+
-
 
+
-
  #footer-box {
+
-
    border:none;
+
-
    display:none;
+
-
    width:100%;
+
-
  }
+
-
 
+
-
  #p-logo {
+
-
    display:none
+
-
  }
+
-
 
+
-
  .firstHeading {
+
-
  display:none;
+
-
  }
+
-
 
+
-
  #bodyContent p{
+
-
    display:none;
+
-
  }
+
-
 
+
-
  html, div, span, applet, object, iframe,
+
-
  h1, h2, h3, h4, h5, h6, p, blockquote, pre,
+
-
  a, abbr, acronym, address, big, cite, code,
+
-
  del, dfn, em, img, ins, kbd, q, s, samp,
+
-
  small, strike, strong, sub, sup, tt, var,
+
-
  b, u, i, center,
+
-
  dl, dt, dd, ol, ul, li,
+
-
  fieldset, form, label, legend,
+
-
  table, caption, tbody, tfoot, thead, tr, th, td,
+
-
  article, aside, canvas, details, embed,
+
-
  figure, figcaption, footer, header, hgroup,
+
-
  menu, nav, output, ruby, section, summary,
+
-
  time, mark, audio, video {
+
-
    margin: 0;
+
-
    padding: 0;
+
-
    border: 0;
+
-
    font-size: 100%;
+
-
    font: inherit;
+
-
    vertical-align: baseline;
+
-
  }
+
-
 
+
-
  #top-section {
+
-
    width: 965px;
+
-
    height: 0;
+
-
    margin: 0 auto;
+
-
    padding: 0;
+
-
    border: none;}
+
-
 
+
-
  .left-menu:hover {
+
-
    background-color: transparent;}
+
-
 
+
-
  #menubar li a {
+
-
    background-color: transparent;}
+
-
 
+
-
  .outer { 
+
-
    position:fixed;
+
-
    width:100%;
+
-
    height:100%;
+
-
  }
+
-
 
+
-
  img.supercoil {
+
-
    width:65%;
+
-
    display: block;
+
-
    margin-left: auto;
+
-
    margin-right: auto }
+
-
 
+
-
  .Heading {
+
-
    position:absolute;
+
-
    display: block;
+
-
  }
+
-
 
+
-
.heading {
+
-
    -webkit-transition: margin-left 0.3s, -webkit-transform 0.3s;
+
-
    transition: margin-left 0.3s;
+
-
}
+
-
 
+
-
.heading:hover {
+
-
    margin-left:30px;
+
-
}
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
.pink_news_block {
+
-
     
+
-
        background: #DEA5A4;
+
-
        border-radius:15px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:3%;padding-bottom:3%;
+
-
width:96%;
+
-
}
+
-
 
+
-
.blue_news_block {
+
-
     
+
-
        background: #ADD8E6;
+
-
        border-radius:15px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:3%;padding-bottom:3%;
+
-
width:96%;
+
-
}
+
-
 
+
-
.orange_news_block1 {
+
-
     
+
-
        background: #FA6800;
+
-
        border-radius:30px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
+
-
width:32%;
+
-
}
+
-
 
+
-
.orange_news_block2 {
+
-
     
+
-
        background: #FA6800;
+
-
        border-radius:30px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
+
-
width:96%;
+
-
}
+
-
 
+
-
.yellow_news_block {
+
-
     
+
-
        background: #FFFF06;
+
-
        border-radius:30px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
+
-
width:27%;
+
-
}
+
-
.white_news_block {
+
-
     
+
-
        background: #fff;
+
-
        border-radius:15px; margin-top:2%; margin-left: 0%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
+
-
width:96%;
+
-
}
+
-
 
+
-
h1 {
+
-
font-size: 120%;
+
font-weight: 400;
font-weight: 400;
font-family:Helvetica;
font-family:Helvetica;
-
margin-bottom: 2%;
+
        color:#4747E5;
-
     
+
        line-height:150%;
-
}
+
-
h1white {
+
-
font-size: 230%;
+
-
font-weight: 400;
+
-
font-family:Helvetica;
+
-
        color:#fff;
+
}
}
 +
</style>
-
h1black {
+
<body>
-
font-size: 230%;
+
  <div class="outer" style="overflow-y: scroll; overflow-x: hidden;">
-
font-weight: 400;
+
-
font-family:Helvetica;
+
-
}
+
-
  </style>
+
<div id="stuff" style="float:left;position:absolute;margin-left:200px;margin-right:100px; margin-top:50px;min-width:645px;">
 +
<img src="https://static.igem.org/mediawiki/2014/0/0f/Real_Bioremediation.jpg" style="position:absolute; width:100%;z-index:-1; border-radius:15px;"/>
 +
<div style="background-color:#D9D9D9; opacity:0.7; z-index:5; Height:75px; width:100%;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;margin-top:10px;">
 +
<div style="background-color:white; opacity:0.9; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#00000; font-weight: 450;"><br><center><font style="opacity:0.7">Bioremediation Homepage</font></center></div>
 +
</div>
 +
<br>
 +
<div style="border-bottom-left-radius:10px;border-bottom-right-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;">
 +
<img src="https://static.igem.org/mediawiki/2014/d/d5/Realisation_bioremediation.png" style="width:35%;margin-left:33%;margin-top:-50px;">
 +
</div>
 +
<div style="background-color:white; border-bottom-left-radius:10px;border-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:-50px;">
 +
<a href="https://static.igem.org/mediawiki/2014/3/3d/OxigemLAB_BOOK.pdf" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/50/OxigemLabbook.png" style="position:absolute;width:6%;margin-left:84%;margin-top:-13%;z-index:10;"></a>
 +
<a href="https://static.igem.org/mediawiki/2014/1/16/Oxigem_LAB_PROTOCOLS.pdf" target="_blank"><img src="https://static.igem.org/mediawiki/2014/a/a4/OxigemProtocols.png" style="position:absolute;width:6%;margin-left:91%;margin-top:-13%;z-index:10;"></a>
 +
<br>
 +
<h1>DCMation: decimating DCM pollution using bioremediation</h1>
 +
Bioremediation is a form of clean waste management that relies on the ability of organisms to break down a wide range of pollutants and toxic wastes that would otherwise harm the environment and potentially pose a threat to human health. Dichloromethane (DCM) is such a toxic waste product, and we have made it our mission to explore and develop a system to safely degrade DCM using bacteria. <br><br>
 +
The three 'DCMation' links below will guide you through our interdisciplinary approach of evaluating and improving the DCM-degrading capacity of the native bacterium, Methylobacterium extorquens DM4. Additionally, these pages explain how and why we have expressed the DCM degradation system in E. coli and P. putida, and how we are adapting them for DCMation.<br><br>
 +
The three 'Microcompartment' pages will take you on a journey of how models of microcompartments designed by our engineers work hand-in-hand with the results that our biochemists obtained in wet-lab experiments. After explaining what microcompartments are and why we use them, we introduce our collaboration with the Melbourne iGEM team and their project, the Star Peptide, which is a possible alternative to the use of microcompartments. <br><br>
 +
<br>
 +
<font style="font-size:medium;font-weight:500;font-style: italic;">DcmA catalysis</font><br>
 +
DcmA is the enzyme responsible for the breakdown of DCM and is expressed in M.extorquens under the control of the regulatory protein DcmR. This enzyme is a member of the glutathione-S-transferase family.
 +
We propose the following plausible mechanism for the catalytic dehalogenation of dichloromethane by DcmA, in which glutathione (RSH) is a co-factor:
-
#list li {
+
<br>
-
list-style-image: url("https://static.igem.org/mediawiki/2014/6/6f/OxigemTick.png"); }
+
<img src="https://static.igem.org/mediawiki/2014/9/9e/OxigemDcmAMechanism.jpg" style="width:70%;margin-left:15%;" />
-
}
+
-
  </style>
+
-
  <script type="text/javascript">
+
-
 
+
-
  function iconleft(x) {
+
-
    x.style.left="6%";
+
-
  }
+
-
  function iconnormal(x) {
+
-
    x.style.left="9%";
+
-
  }
+
-
$(document).ready(function(){
+
-
  $("#arrowr").click(function(){
+
-
    $("#grey").animate({right:'-175px'}, "fast");
+
-
  });
+
-
  $("#arrowl").click(function(){
+
-
    $("#grey").animate({right:'-120px'}, "fast");
+
-
  });
+
-
  $("#modelling").hover(function(){
+
-
    $("#modelling").animate({opacity:'0.95'}, "fast");
+
-
    $("#modelling1").css({display:'inline'});
+
-
  });
+
-
  $("#modelling").mouseout(function(){
+
-
    $("#modelling").animate({opacity:'0.75'}, "fast");
+
-
    $("#modelling1").css({display:'none'});
+
-
  });
+
-
  $("#achievements").hover(function(){
+
-
    $("#achievements").animate({opacity:'0.95'}, "fast");
+
-
    $("#achievements1").css({display:'inline'});
+
-
  });
+
-
  $("#achievements").mouseout(function(){
+
-
    $("#achievements").animate({opacity:'0.75'}, "fast");
+
-
    $("#achievements1").css({display:'none'});
+
-
  });
+
-
  $("#igemcomm").hover(function(){
+
-
    $("#igemcomm").animate({opacity:'0.95'}, "fast");
+
-
    $("#igemcomm1").css({display:'inline'});
+
-
  });
+
-
  $("#igemcomm").mouseout(function(){
+
-
    $("#igemcomm").animate({opacity:'0.75'}, "fast");
+
-
    $("#igemcomm1").css({display:'none'});
+
-
  });
+
-
  $("#safety").hover(function(){
+
-
    $("#safety").animate({opacity:'0.95'}, "fast");
+
-
    $("#safety1").css({display:'inline'});
+
-
  });
+
-
  $("#safety").mouseout(function(){
+
-
    $("#safety").animate({opacity:'0.75'}, "fast");
+
-
    $("#safety1").css({display:'none'});
+
-
  });
+
-
  $("#team").hover(function(){
+
-
    $("#team").animate({opacity:'0.95'}, "fast");
+
-
    $("#team1").css({display:'inline'});
+
-
  });
+
-
  $("#team").mouseout(function(){
+
-
    $("#team").animate({opacity:'0.75'}, "fast");
+
-
    $("#team1").css({display:'none'});
+
-
  });
+
-
  $("#dcmation").hover(function(){
+
-
    $("#dcmation").animate({opacity:'0.95'}, "fast");
+
-
    $("#dcmation1").css({display:'inline'});
+
-
  });
+
-
  $("#dcmation").mouseout(function(){
+
-
    $("#dcmation").animate({opacity:'0.75'}, "fast");
+
-
    $("#dcmation1").css({display:'none'});
+
-
  });
+
-
  $("#interlab").hover(function(){
+
-
    $("#interlab").animate({opacity:'0.95'}, "fast");
+
-
    $("#interlab1").css({display:'inline'});
+
-
  });
+
-
  $("#interlab").mouseout(function(){
+
-
    $("#interlab").animate({opacity:'0.75'}, "fast");
+
-
    $("#interlab1").css({display:'none'});
+
-
  });
+
-
  $("#policy").hover(function(){
+
-
    $("#policy").animate({opacity:'0.95'}, "fast");
+
-
    $("#policy1").css({display:'inline'});
+
-
  });
+
-
  $("#policy").mouseout(function(){
+
-
    $("#policy").animate({opacity:'0.75'}, "fast");
+
-
    $("#policy1").css({display:'none'});
+
-
  });
+
-
});
+
-
 
+
-
 
+
-
jQuery(document).ready(function(){
+
-
    jQuery('#tb1').live('click', function(event) {       
+
-
        jQuery('#b1').toggle('show');
+
-
    });
+
-
    jQuery('#tb2').live('click', function(event) {       
+
-
        jQuery('#b2').toggle('show');
+
-
    });
+
-
    jQuery('#tb3').live('click', function(event) {       
+
-
        jQuery('#b3').toggle('show');
+
-
    });
+
-
    jQuery('#tb4').live('click', function(event) {       
+
-
        jQuery('#b4').toggle('show');
+
-
    });
+
-
    jQuery('#tb5').live('click', function(event) {       
+
-
        jQuery('#b5').toggle('show');
+
-
    });
+
-
    jQuery('#ts1').live('click', function(event) {       
+
-
        jQuery('#s1').toggle('show');
+
-
    });
+
-
    jQuery('#ts2').live('click', function(event) {       
+
-
        jQuery('#s2').toggle('show');
+
-
    });
+
-
    jQuery('#ts3').live('click', function(event) {       
+
-
        jQuery('#s3').toggle('show');
+
-
    });
+
-
    jQuery('#ts4').live('click', function(event) {       
+
-
        jQuery('#s4').toggle('show');
+
-
    });
+
-
    jQuery('#tg1').live('click', function(event) {       
+
-
        jQuery('#g1').toggle('show');
+
-
    });
+
-
    jQuery('#tg2').live('click', function(event) {       
+
-
        jQuery('#g2').toggle('show');
+
-
    });
+
-
    jQuery('#tg3').live('click', function(event) {       
+
-
        jQuery('#g3').toggle('show');
+
-
    });
+
-
});
+
-
 
+
-
 
+
-
function startup(){
+
-
  $("#sidebarblue").animate({marginLeft:'+=250px'}, 1000);
+
-
  $("#sidebarlogo").animate({marginLeft:'+=250px'}, 1000);
+
-
}
+
-
function startup1(){
+
-
  $(".1").animate({marginLeft:'+=250px'}, 1000);
+
-
}
+
-
function startup2(){
+
-
  $(".2").animate({marginLeft:'+=250px'}, 1000);
+
-
}
+
-
function startup3(){
+
-
  $(".3").animate({marginLeft:'+=250px'}, 1000);
+
-
}
+
-
function startup4(){
+
-
  $(".4").animate({marginLeft:'+=250px'}, 1000);
+
-
}
+
-
 
+
-
 
+
-
 
+
-
$(document).ready(function(){
+
-
  setTimeout(function() {
+
-
    startup();
+
-
  }, 1500);
+
-
  setTimeout(function() {
+
-
    startup1();
+
-
  }, 1520);
+
-
  setTimeout(function() {
+
-
    startup2();
+
-
  }, 1560);
+
-
  setTimeout(function() {
+
-
    startup3();
+
-
  }, 1640);
+
-
  setTimeout(function() {
+
-
    startup4();
+
-
  }, 1750);
+
-
});
+
-
 
+
-
  </script>
+
-
 
+
-
  <title></title>
+
-
</head>
+
-
 
+
-
<body>
+
-
  <div class="outer" style="overflow-y: scroll; overflow-x: hidden;">
+
-
 
+
-
 
+
-
 
+
-
<div id="stuff" style="float:left;position:absolute;margin-left:200px;margin-right:100px; margin-top:50px;">
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2014/0/08/Oxigemradcamcrop.jpg" style="position:absolute; width:100%;min-width:100%;z-index:-1; border-radius:15px;"/>
+
-
 
+
-
<div style="background-color:#D9D9D9; opacity:0.7; z-index:5; margin-right:auto;margin-left:auto; Height:75px; width:60%;min-width:300px;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;">
 
-
<div style="background-color:white; opacity:0.7; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;min-width:300px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#596C8A; font-weight: 450;"><br>Bioremediation</div>
 
</div>
</div>
-
<br><br>
 
-
 
-
 
-
 
-
 
-
<div class="white_news_block">
 
-
<h1>Bioremediation</h1>
 
-
BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES BIOREMEDIATION INTRODUCTION PLUS QUOTES
 
-
</div>
 
-
 
Line 435: Line 60:
-
<img src="https://static.igem.org/mediawiki/2014/7/76/Oxford_Micro_title.png" style="float:right;position:relative; width:48%;" />
+
<img src="https://static.igem.org/mediawiki/2014/7/76/Oxford_Micro_title.png" style="float:right;position:relative; width:48%; margin-left:4%;" />
<img src="https://static.igem.org/mediawiki/2014/f/fa/Oxford_DCMation_title.png" style="float:left;position:relative; width:48%;" />
<img src="https://static.igem.org/mediawiki/2014/f/fa/Oxford_DCMation_title.png" style="float:left;position:relative; width:48%;" />
Line 458: Line 83:
<a href="https://2014.igem.org/Team:Oxford/bioremediation">
<a href="https://2014.igem.org/Team:Oxford/bioremediation">
-
<img src="https://static.igem.org/mediawiki/2014/b/b2/Oxford_bioremediation_title_dark.png" style="float:left;position:relative; width:80%;margin-top:1.5%;margin-left:10%;" /></a>
+
<img src="https://static.igem.org/mediawiki/2014/b/b2/Oxford_bioremediation_title_dark.png" style="float:left;position:relative; width:50%;margin-top:1.5%;margin-left:25%;margin-right:25%;" /></a>
 +
<br><br><br><br><br>
 +
Oxford iGEM 2014
-
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 
-
</div>
 
</div>
</div>
Line 468: Line 93:
 +
<br>
Line 473: Line 99:
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
<div id="sponsors" style="height:10%; width:100%;"></div>
 
-
 
-
     
 
-
</div>
 
-
 
-
<img id="sidebarblue" src="https://static.igem.org/mediawiki/2014/e/ec/OxigemsidebarBlue.png" style="float:left;position:fixed;margin-left:-250px;height:100%;" />
 
-
 
-
<img class="1" src="https://static.igem.org/mediawiki/2014/a/a5/OxigemDotsDCMation.png" style="opacity:0.9;float:left;position:fixed;margin-left:-250px; top:31.6%; height:17.6%;" />
 
-
<img class="2" src="https://static.igem.org/mediawiki/2014/0/06/OxigemDotsinterlab.png" style="opacity:0.9;float:left;position:fixed;margin-left:-250px; top:18.3%; height:15.125%;" />
 
-
<img class="3" src="https://static.igem.org/mediawiki/2014/e/ee/OxigemDotsP%26P.png" style="opacity:0.9;float:left;position:fixed;margin-left:-250px; top:9%; height:12.75%;" />
 
-
<img class="4" src="https://static.igem.org/mediawiki/2014/c/ca/OxigemDotsTeam.png" style="float:left;position:fixed;margin-left:-250px; top:0%; height:12.625%;" />
 
-
<img class="1" src="https://static.igem.org/mediawiki/2014/f/f5/OxigemDotsModelling.png" style="opacity:0.8;float:left;position:fixed;margin-left:-250px; top:50.6%; height:17.75%;" />
 
-
<img class="2" src="https://static.igem.org/mediawiki/2014/8/87/OxigemDotsAchievements.png" style="opacity:0.9;float:left;position:fixed;margin-left:-250px; top:66.45%; height:13.28%;" />
 
-
<img class="3" src="https://static.igem.org/mediawiki/2014/7/7b/OxigemDotsiGEMcomm.png" style="opacity:0.9;float:left;position:fixed;margin-left:-250px; top:78%; height:12.5%;" />
 
-
<img class="4" src="https://static.igem.org/mediawiki/2014/0/0e/OxigemDotsSafety.png" style="float:left;position:fixed;margin-left:-250px; top:88.15%; height:11.9%;" />
 
-
 
-
<a href="https://2014.igem.org/Team:Oxford"><img id="sidebarlogo" class="oxigem" src="https://static.igem.org/mediawiki/2014/a/a4/Oxigemsidebarlogo.png" style="float:left;position:fixed;margin-left:-250px; top:42.5%; height:15%;"/></a>
 
-
 
-
<img src="https://static.igem.org/mediawiki/2014/3/37/OxigemDotModelling.png" style="float:left;position:fixed; top:59.3%; height:4.125%;" />
 
-
<img src="https://static.igem.org/mediawiki/2014/0/05/OxigemDotAchievements.png" style="float:left;position:fixed; top:71%; height:4%;" />
 
-
<img src="https://static.igem.org/mediawiki/2014/7/77/OxigemDotiGEMcomm.png" style="float:left;position:fixed; top:81.5%; height:4.375%;" />
 
-
<img src="https://static.igem.org/mediawiki/2014/2/27/OxigemDotSafety.png" style="float:left;position:fixed; top:93%; height:4.125%;" />
 
-
<img src="https://static.igem.org/mediawiki/2014/e/e7/OxigemDotDCMation.png" style="float:left;position:fixed; top:37%; height:4.125%;" />
 
-
<img src="https://static.igem.org/mediawiki/2014/9/94/OxigemDotInterlab.png" style="float:left;position:fixed; top:25%; height:4.125%;" />
 
-
<img src="https://static.igem.org/mediawiki/2014/1/19/OxigemDotP%26P.png" style="float:left;position:fixed; top:13.5%; height:4.375%;" />
 
-
<img src="https://static.igem.org/mediawiki/2014/c/cc/OxigemDotTeam.png" style="float:left;position:fixed; top:3%; height:4.375%;" />
 
-
 
-
<img id="modelling1" src="https://static.igem.org/mediawiki/2014/9/93/OxigemDotModelling1.png" style="display:none;float:left;position:fixed; top:59.3%; height:4.125%;" />
 
-
<img id="achievements1" src="https://static.igem.org/mediawiki/2014/0/0f/OxigemDotAchievements1.png" style="display:none;float:left;position:fixed; top:71%; height:4%;" />
 
-
<img id="igemcomm1" src="https://static.igem.org/mediawiki/2014/a/a8/OxigemDotiGEMcomm1.png" style="display:none;float:left;position:fixed; top:81.5%; height:4.375%;" />
 
-
<img id="safety1" src="https://static.igem.org/mediawiki/2014/e/ee/OxigemDotSafety1.png" style="display:none;float:left;position:fixed; top:93%; height:4.125%;" />
 
-
<img id="dcmation1" src="https://static.igem.org/mediawiki/2014/d/db/OxigemDotDCMation1.png" style="display:none;float:left;position:fixed; top:37%; height:4.125%;" />
 
-
<img id="interlab1" src="https://static.igem.org/mediawiki/2014/5/52/OxigemDotInterlab1.png" style="display:none;float:left;position:fixed; top:25%; height:4.125%;" />
 
-
<img id="policy1" src="https://static.igem.org/mediawiki/2014/b/bf/OxigemDotP%26P1.png" style="display:none;float:left;position:fixed; top:13.5%; height:4.375%;" />
 
-
<img id="team1" src="https://static.igem.org/mediawiki/2014/c/c5/OxigemDotTeam1.png" style="display:none;float:left;position:fixed; top:3%; height:4.375%;" />
 
-
 
-
<a href="#"><img class="heading" id="modelling" src="https://static.igem.org/mediawiki/2014/e/e0/OxigemSidebarModelling.png" style="opacity:0.8;float:left;position:fixed; top:59%; height:4.5%;"  /></a>
 
-
<a href="#"><img class="heading" id="achievements" src="https://static.igem.org/mediawiki/2014/4/4a/OxigemSidebarAchievements.png" style="opacity:0.75;float:left;position:fixed; top:71%; height:4%;" /></a>
 
-
<a href="#"><img class="heading" id="igemcomm" src="https://static.igem.org/mediawiki/2014/8/83/OxigemSidebariGEMcomm.png" style="opacity:0.8;float:left;position:fixed; top:81.1%; height:5.375%;" /></a>
 
-
<a href="https://2014.igem.org/Team:Oxford/safety"><img class="heading" id="safety" src="https://static.igem.org/mediawiki/2014/3/3d/OxigemSidebarSafety.png" style="opacity:0.75;float:left;position:fixed; top:92.9%; height:4.375%;" /></a>
 
-
<a href="#"><img class="heading" id="dcmation" src="https://static.igem.org/mediawiki/2014/1/18/OxigemSidebarDCMation.png" style="opacity:0.8;float:left;position:fixed; top:36%; height:5.75%;" /></a>
 
-
<a href="#"><img class="heading" id="interlab" src="https://static.igem.org/mediawiki/2014/e/e2/OxigemSidebarInterlab.png" style="opacity:0.8;float:left;position:fixed; top:24.4%; height:5.25%;" /></a>
 
-
<a href="https://2014.igem.org/Team:Oxford/policy_and_practices"><img class="heading" id="policy" src="https://static.igem.org/mediawiki/2014/7/7b/OxigemSidebarP%26P.png" style="opacity:0.8;float:left;position:fixed; top:13.2%; height:4.875%;" /></a>
 
-
<a href="https://2014.igem.org/Team:Oxford/team"><img class="heading" id="team" src="https://static.igem.org/mediawiki/2014/3/3b/OxigemSidebarTeam.png" style="opacity:0.8;float:left;position:fixed; top:3%; height:4.625%;" /></a>
 
-
 
-
 
-
 
-
 
-
 
-
 
-
<img src="https://static.igem.org/mediawiki/2014/6/60/OxigemAchievements2.png" style="position:fixed; display:none; height:4%; top:37%; left:2%;" />
 
-
 
-
 
-
  <div class="iGEMlogo" style="width:6.5%; position:absolute; top:0.7%; right:0.5%; min-width:50px;">
 
-
    <a href="https://2014.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/56/OxigemiGEMgrey.png" style="width:100%"/></a>
 
-
  </div>
 
-
  <a href="#"><img id="arrowl" src="https://static.igem.org/mediawiki/2014/1/1d/OxigemArrowleft.png" style="position:fixed; width:50px; top:23%;right:0px; opacity:0.6; z-index:4;" /></a>
 
-
  <div id="grey" style="width:190px; position:fixed; top:23%; right:-175px; z-index:4;">
 
-
    <img src="https://static.igem.org/mediawiki/2014/0/0d/OxigemGrey.png" style="position:relative;width:100%;" />
 
-
    <a href="#"><img id="arrowr" src="https://static.igem.org/mediawiki/2014/0/03/OxigemArrowr.png" style="position:absolute;width:14%;top:5%;left:10%;"></a>
 
-
    <div class="fbicon" style="position:absolute;width:23%; top:20%; left:9%;" onmouseover=
 
-
      "iconleft(this)" onmouseout="iconnormal(this)" border="0"><a href="https://www.facebook.com/oxfordigem" target="_blank" style="background-image: none;"><img src="https://static.igem.org/mediawiki/2014/8/88/OxigemIconfacebook.png" style="width:100%;" /></a>
 
-
    </div> 
 
-
  <div class="twicon" style="position:absolute;width:23%; top:35%; left:9%;" onmouseover=
 
-
      "iconleft(this)" onmouseout="iconnormal(this)" border="0">
 
-
    <a href="https://twitter.com/OxfordiGEM" target="_blank" style="background-image: none;"><img src="https://static.igem.org/mediawiki/2014/3/3d/OxigemIgemtwitter.png" style="width:100%;" /></a>
 
-
  </div>
 
-
  <div class="yticon" style="position:absolute;width:23%; top:50%; left:9%;" onmouseover=
 
-
      "iconleft(this)" onmouseout="iconnormal(this)" border="0">
 
-
    <a href="https://www.youtube.com/channel/UCx1QJHqQcZ09aT97V2f4Xdw" target="_blank" style="background-image: none;"><img src="https://static.igem.org/mediawiki/2014/e/ea/OxigemIconyoutube.png" style="width:100%;" /></a>
 
-
  </div>
 
-
  <div class="oxicon" style="position:absolute;width:23%; top:65%; left:9%;" onmouseover=
 
-
      "iconleft(this)" onmouseout="iconnormal(this)" border="0">
 
-
    <a href="http://www.ox.ac.uk" target="_blank"><img src="https://static.igem.org/mediawiki/2014/8/8d/Iconoxford.png" style="width:100%;" /></a>
 
-
  </div>
 
-
  <div class="mailicon" style="position:absolute;width:23%; top:80%; left:9%;" onmouseover=
 
-
      "iconleft(this)" onmouseout="iconnormal(this)" border="0" >
 
-
    <a href="mailto:oxfordigem@bioch.ox.ac.uk" target="_blank" style="background-image: none;"><img src="https://static.igem.org/mediawiki/2014/3/35/OxigemIconmail.png" style="width:100%;" /></a>
 
-
  </div>
 
</body>
</body>
</html>
</html>
 +
 +
{{Team:Oxford/templates/footer}}

Latest revision as of 02:17, 18 October 2014


Bioremediation Homepage


DCMation: decimating DCM pollution using bioremediation

Bioremediation is a form of clean waste management that relies on the ability of organisms to break down a wide range of pollutants and toxic wastes that would otherwise harm the environment and potentially pose a threat to human health. Dichloromethane (DCM) is such a toxic waste product, and we have made it our mission to explore and develop a system to safely degrade DCM using bacteria.

The three 'DCMation' links below will guide you through our interdisciplinary approach of evaluating and improving the DCM-degrading capacity of the native bacterium, Methylobacterium extorquens DM4. Additionally, these pages explain how and why we have expressed the DCM degradation system in E. coli and P. putida, and how we are adapting them for DCMation.

The three 'Microcompartment' pages will take you on a journey of how models of microcompartments designed by our engineers work hand-in-hand with the results that our biochemists obtained in wet-lab experiments. After explaining what microcompartments are and why we use them, we introduce our collaboration with the Melbourne iGEM team and their project, the Star Peptide, which is a possible alternative to the use of microcompartments.


DcmA catalysis
DcmA is the enzyme responsible for the breakdown of DCM and is expressed in M.extorquens under the control of the regulatory protein DcmR. This enzyme is a member of the glutathione-S-transferase family. We propose the following plausible mechanism for the catalytic dehalogenation of dichloromethane by DcmA, in which glutathione (RSH) is a co-factor:





Oxford iGEM 2014