Team:Oxford/InterlabDevices
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- | + | <font style="font-weight:bold">I. Using the DNA distribution kit to extract devices 1-3:</font> | |
- | <img src="https://static.igem.org/mediawiki/2014/5/5f/Devices.png" style="width:60%;margin-left:20%" /><br> | + | <img src="https://static.igem.org/mediawiki/2014/5/5f/Devices.png" style="width:60%;margin-left:20%" /><br><br> |
We filled the 4 wells we needed with 10 uL of dH2O and let it sit for about 10 minutes to resuspend. We transformed the resuspended DNA into chemically competent <i>E.coli cells</i> (DH5) and plated them on an agar plate with kanamycin (plate for device 1) and chloramphenicol (plates for devices 2a, 2b/3b, and 3a) using the transformation into chemically competent E. coli cells protocol (however, we used 200 uL of competent cells per transformation rather than 100uL). | We filled the 4 wells we needed with 10 uL of dH2O and let it sit for about 10 minutes to resuspend. We transformed the resuspended DNA into chemically competent <i>E.coli cells</i> (DH5) and plated them on an agar plate with kanamycin (plate for device 1) and chloramphenicol (plates for devices 2a, 2b/3b, and 3a) using the transformation into chemically competent E. coli cells protocol (however, we used 200 uL of competent cells per transformation rather than 100uL). | ||
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<font style="font-weight:bold">IV. Sequencing Results:</font><br> | <font style="font-weight:bold">IV. Sequencing Results:</font><br> | ||
We received sequencing results that confirmed the sequences of the parts we were using from the DNA-distribution kit, including the two point-mutations in part 3A. Shown below are the sequence alignments of the sequence we expected (top strand) and the sequencing results (bottom) of parts 2A (left) and 3A (right):</p> | We received sequencing results that confirmed the sequences of the parts we were using from the DNA-distribution kit, including the two point-mutations in part 3A. Shown below are the sequence alignments of the sequence we expected (top strand) and the sequencing results (bottom) of parts 2A (left) and 3A (right):</p> | ||
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- | + | <img src="https://static.igem.org/mediawiki/2014/9/90/Oxigemsequence_alignment.jpg" style="width:80%; margin-left:10%;margin-top:10px;margin-bottom:10px;" | |
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Latest revision as of 22:53, 17 October 2014