Team:Oxford

From 2014.igem.org

(Difference between revisions)
 
(38 intermediate revisions not shown)
Line 3: Line 3:
<html>
<html>
-
 
-
<style>
 
-
img.one {
 
-
opacity:0.9;
 
-
}
 
-
img.one:hover {
 
-
opacity:1;
 
-
}
 
-
img.two {
 
-
opacity:0.9;
 
-
}
 
-
img.two:hover {
 
-
opacity:1;
 
-
}
 
-
img.three {
 
-
opacity:0.9;
 
-
}
 
-
img.three:hover {
 
-
opacity:1;
 
-
}
 
-
</style>
 
<body>
<body>
Line 68: Line 47:
       <img id="achievementsblue" src="https://static.igem.org/mediawiki/2014/9/9e/OxigemAchievementsblue.png" style="opacity:0; position:absolute; width:17.41328%; left: 49.5%; top: 61%;">
       <img id="achievementsblue" src="https://static.igem.org/mediawiki/2014/9/9e/OxigemAchievementsblue.png" style="opacity:0; position:absolute; width:17.41328%; left: 49.5%; top: 61%;">
       <img id="DCMationblue" src="https://static.igem.org/mediawiki/2014/2/2f/OxigemDCMationblue.png" style="opacity:0;position:absolute; width:17.94096%; left: 31.95%; top: 60.6%;">
       <img id="DCMationblue" src="https://static.igem.org/mediawiki/2014/2/2f/OxigemDCMationblue.png" style="opacity:0;position:absolute; width:17.94096%; left: 31.95%; top: 60.6%;">
-
   
 
     <div id="abstract" style="position: absolute; width:0%; height:100%; top: 0%; left: 50%; background-color: #f5f5f5; z-index:10; border-radius:15px;">
     <div id="abstract" style="position: absolute; width:0%; height:100%; top: 0%; left: 50%; background-color: #f5f5f5; z-index:10; border-radius:15px;">
<a href="#"><img id="x" src="https://static.igem.org/mediawiki/2014/7/7f/Oxigemx.png" onclick="hideabstract()" style="position:absolute;opacity:0.6;right:0.9%;top:1.5%;display:none;" /></a>
<a href="#"><img id="x" src="https://static.igem.org/mediawiki/2014/7/7f/Oxigemx.png" onclick="hideabstract()" style="position:absolute;opacity:0.6;right:0.9%;top:1.5%;display:none;" /></a>
-
 
       <div id="abstract2" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:scroll;overflow-x:visible;">
       <div id="abstract2" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:scroll;overflow-x:visible;">
       <div class="inner" style="width:100%;margin-left:auto;margin-right:auto;height:100%;position:absolute;">
       <div class="inner" style="width:100%;margin-left:auto;margin-right:auto;height:100%;position:absolute;">
<img class="DCMationgif" style="margin-left:5%;" src="https://static.igem.org/mediawiki/2014/8/81/DCMation1.png" width="90%" align="middle" alt="" onclick='javascript:(this.src=="https://static.igem.org/mediawiki/2014/8/81/DCMation1.png"?this.src="https://static.igem.org/mediawiki/2014/e/e2/OxigemDCMationgif.gif":"")' />
<img class="DCMationgif" style="margin-left:5%;" src="https://static.igem.org/mediawiki/2014/8/81/DCMation1.png" width="90%" align="middle" alt="" onclick='javascript:(this.src=="https://static.igem.org/mediawiki/2014/8/81/DCMation1.png"?this.src="https://static.igem.org/mediawiki/2014/e/e2/OxigemDCMationgif.gif":"")' />
-
 
University of Oxford’s first iGEM team presents: DCMation, a novel bioremediation approach whose applications are limited only by the versatility of bacterial metabolism. OxiGEM are tackling environmental pollution by developing a user-friendly device for the detection & degradation of the hazardous yet indispensable solvent dichloromethane (DCM), to illustrate.  
University of Oxford’s first iGEM team presents: DCMation, a novel bioremediation approach whose applications are limited only by the versatility of bacterial metabolism. OxiGEM are tackling environmental pollution by developing a user-friendly device for the detection & degradation of the hazardous yet indispensable solvent dichloromethane (DCM), to illustrate.  
-
<br><br>
+
<br>
Inspired by the DCM degradation pathway of <font style="font-style: italic;">M. extorquens</font> DM4, our project is driven and refined by the dialogue between modelling simulations and experimental data. Bioremediation is optimised by expressing the DCM degrading system in host strains, along with microcompartments to accelerate the reaction and minimise toxic intermediates. Our biosensor is tuned to our characterisation and improvements of the catalytic efficiency of the system, while incorporation of the bacteria into novel diffusion-limiting biopolymeric beads ensures safe and rapid degradation.
Inspired by the DCM degradation pathway of <font style="font-style: italic;">M. extorquens</font> DM4, our project is driven and refined by the dialogue between modelling simulations and experimental data. Bioremediation is optimised by expressing the DCM degrading system in host strains, along with microcompartments to accelerate the reaction and minimise toxic intermediates. Our biosensor is tuned to our characterisation and improvements of the catalytic efficiency of the system, while incorporation of the bacteria into novel diffusion-limiting biopolymeric beads ensures safe and rapid degradation.
<br>
<br>
This all-round modular design and scalability make DCMation ideal for extension to the disposal of many harmful substances. Explore our wiki for more!
This all-round modular design and scalability make DCMation ideal for extension to the disposal of many harmful substances. Explore our wiki for more!
 +
 +
       </div>
       </div>
       </div>
       </div>
       <div id="menu1" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:visible;overflow-x:visible;">
       <div id="menu1" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:visible;overflow-x:visible;">
-
     
 
-
 
<img src="https://static.igem.org/mediawiki/2014/7/78/OxigemBiosensor1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
<img src="https://static.igem.org/mediawiki/2014/7/78/OxigemBiosensor1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
<a href="#"><img id="next1" src="https://static.igem.org/mediawiki/2014/7/73/OxigemNext.png" style="position:absolute;width:3%;margin-left:101.5%;margin-top:27%;"  /></a>
<a href="#"><img id="next1" src="https://static.igem.org/mediawiki/2014/7/73/OxigemNext.png" style="position:absolute;width:3%;margin-left:101.5%;margin-top:27%;"  /></a>
-
 
+
<a href="https://2014.igem.org/Team:Oxford/biosensor"><img class="zero" src="https://static.igem.org/mediawiki/2014/a/af/Oxigem1.0.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
-
<a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation"><img class="one" src="https://static.igem.org/mediawiki/2014/f/f8/Oxigem1.1.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
+
<a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation"><img class="one" src="https://static.igem.org/mediawiki/2014/f/f8/Oxigem1.1.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/biosensor_construction"><img class="two" src="https://static.igem.org/mediawiki/2014/8/84/Oxigem1.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/biosensor_construction"><img class="two" src="https://static.igem.org/mediawiki/2014/8/84/Oxigem1.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/biosensor_optimisation"><img class="three" src="https://static.igem.org/mediawiki/2014/d/df/Oxigem1.3.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/biosensor_optimisation"><img class="three" src="https://static.igem.org/mediawiki/2014/d/df/Oxigem1.3.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
-
 
       </div>
       </div>
       <div id="menu2" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:visible;overflow-x:visible;">
       <div id="menu2" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:visible;overflow-x:visible;">
-
 
<img src="https://static.igem.org/mediawiki/2014/e/e8/OxigemBioremediation1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
<img src="https://static.igem.org/mediawiki/2014/e/e8/OxigemBioremediation1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
<a href="#"><img id="next2" src="https://static.igem.org/mediawiki/2014/7/73/OxigemNext.png" style="position:absolute;width:3%;margin-left:101.5%;margin-top:27%;"  /></a>
<a href="#"><img id="next2" src="https://static.igem.org/mediawiki/2014/7/73/OxigemNext.png" style="position:absolute;width:3%;margin-left:101.5%;margin-top:27%;"  /></a>
<a href="#"><img id="back1" src="https://static.igem.org/mediawiki/2014/0/0c/OxigemBack.png" style="position:absolute;width:3%;margin-left:-4.5%;margin-top:27%;"  /></a>
<a href="#"><img id="back1" src="https://static.igem.org/mediawiki/2014/0/0c/OxigemBack.png" style="position:absolute;width:3%;margin-left:-4.5%;margin-top:27%;"  /></a>
-
 
+
<a href="https://2014.igem.org/Team:Oxford/bioremediation"><img class="one" src="https://static.igem.org/mediawiki/2014/d/d3/Oxigem2.0.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
-
<a href="https://2014.igem.org/Team:Oxford/how_much_can_we_degrade?"><img class="one" src="https://static.igem.org/mediawiki/2014/2/21/Oxigem2.1.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
+
<a href="https://2014.igem.org/Team:Oxford/how_much_can_we_degrade?"><img class="two" src="https://static.igem.org/mediawiki/2014/2/21/Oxigem2.1.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/what_are_microcompartments?"><img class="two" src="https://static.igem.org/mediawiki/2014/b/bb/Oxigem2.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/what_are_microcompartments?"><img class="two" src="https://static.igem.org/mediawiki/2014/b/bb/Oxigem2.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
       </div>
       </div>
       <div id="menu3" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:visible;overflow-x:visible;">
       <div id="menu3" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:visible;overflow-x:visible;">
-
 
<img src="https://static.igem.org/mediawiki/2014/1/1b/OxigemRealisation1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
<img src="https://static.igem.org/mediawiki/2014/1/1b/OxigemRealisation1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
<a href="#"><img id="back2" src="https://static.igem.org/mediawiki/2014/0/0c/OxigemBack.png" style="position:absolute;width:3%;margin-left:-4.5%;margin-top:27%;"  /></a>
<a href="#"><img id="back2" src="https://static.igem.org/mediawiki/2014/0/0c/OxigemBack.png" style="position:absolute;width:3%;margin-left:-4.5%;margin-top:27%;"  /></a>
-
 
-
 
<a href="https://2014.igem.org/Team:Oxford/biosensor_realisation"><img class="one" src="https://static.igem.org/mediawiki/2014/f/f6/Oxigem3.1.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/biosensor_realisation"><img class="one" src="https://static.igem.org/mediawiki/2014/f/f6/Oxigem3.1.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/realisation_bioremediation"><img class="two" src="https://static.igem.org/mediawiki/2014/1/1b/Oxigem3.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/realisation_bioremediation"><img class="two" src="https://static.igem.org/mediawiki/2014/1/1b/Oxigem3.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/biopolymer_containment"><img class="three" src="https://static.igem.org/mediawiki/2014/9/91/Oxigem3.3.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
<a href="https://2014.igem.org/Team:Oxford/biopolymer_containment"><img class="three" src="https://static.igem.org/mediawiki/2014/9/91/Oxigem3.3.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
       </div>
       </div>
-
 
-
 
     </div>
     </div>
-
 
       <img id="down" src="https://static.igem.org/mediawiki/2014/1/1c/OxigemDown.png" style="position: absolute; width:65%; top: 0; left: 17%;">
       <img id="down" src="https://static.igem.org/mediawiki/2014/1/1c/OxigemDown.png" style="position: absolute; width:65%; top: 0; left: 17%;">
       <a href="#"><img id="split" src="https://static.igem.org/mediawiki/2014/2/21/OxigemBlank.png" onclick="showabstract()" style="position: absolute; width:24%; top:35%; left: 38%;" /></a>
       <a href="#"><img id="split" src="https://static.igem.org/mediawiki/2014/2/21/OxigemBlank.png" onclick="showabstract()" style="position: absolute; width:24%; top:35%; left: 38%;" /></a>
-
   
 
     </div>
     </div>
 +
       <div class="Heading" id="DCMationheading" style="top: 72.2%; left: 34.8%; width:11%;" onclick="DCMation()" onmouseover=
       <div class="Heading" id="DCMationheading" style="top: 72.2%; left: 34.8%; width:11%;" onclick="DCMation()" onmouseover=
       "DCMationbig(this)" onmouseout="DCMationnormal(this)" border="0"><a href="#"><img src=
       "DCMationbig(this)" onmouseout="DCMationnormal(this)" border="0"><a href="#"><img src=
Line 172: Line 140:
           <div class="Heading" style="top: 14.2%; left: 31%; width:38%;" onmouseover="Subheadingbig(this)" onmouseout=
           <div class="Heading" style="top: 14.2%; left: 31%; width:38%;" onmouseover="Subheadingbig(this)" onmouseout=
           "Subheadingnormal(this)" border="0">
           "Subheadingnormal(this)" border="0">
-
             <a href="#"><img id="openabstract" src=
+
             <a href="#"><img id="openabstract" onclick="showabstract()"  src=
-
             "https://static.igem.org/mediawiki/2014/1/1f/OxigemAbstract.png" onclick="showabstract()" style="width: 100%;"></a>
+
             "https://static.igem.org/mediawiki/2014/1/1f/OxigemAbstract.png" style="width: 100%;"></a>
           </div>
           </div>
           <div class="Heading" style="top: 33%; left: 30.9%; width:38.2%;" onmouseover="Subheadingbig(this)" onmouseout=
           <div class="Heading" style="top: 33%; left: 30.9%; width:38.2%;" onmouseover="Subheadingbig(this)" onmouseout=
Line 244: Line 212:
         "left:25%; width:0%; position:absolute; margin-top:19%;">
         "left:25%; width:0%; position:absolute; margin-top:19%;">
           <img src="https://static.igem.org/mediawiki/2014/e/e9/OxigemiGEMcommbg.png" style="width: 100%;">
           <img src="https://static.igem.org/mediawiki/2014/e/e9/OxigemiGEMcommbg.png" style="width: 100%;">
-
           <div class="Heading" style="top: 60%; left: 29%; width:46%;" onmouseover="Collabbig(this)" onmouseout=
+
           <div class="Heading" style="top: 61.1%; left: 34.3%; width:43%;" onmouseover="Collabbig(this)" onmouseout=
           "Collabnormal(this)" border="0">
           "Collabnormal(this)" border="0">
             <a href="https://2014.igem.org/Team:Oxford/alternatives_to_microcompartments"><img src=
             <a href="https://2014.igem.org/Team:Oxford/alternatives_to_microcompartments"><img src=
             "https://static.igem.org/mediawiki/2014/0/0c/OxigemCollaboration.png" style="width: 100%;"></a>
             "https://static.igem.org/mediawiki/2014/0/0c/OxigemCollaboration.png" style="width: 100%;"></a>
           </div>
           </div>
-
           <div class="Heading" style="top: 41%; left: 53%; width:29.5%;" onmouseover="Eventsbig(this)" onmouseout=
+
           <div class="Heading" style="top: 17.5%; left: 54.5%; width:29.5%;" onmouseover="Eventsbig(this)" onmouseout=
           "Eventsnormal(this)" border="0">
           "Eventsnormal(this)" border="0">
             <a href="https://2014.igem.org/Team:Oxford/Events"><img src=
             <a href="https://2014.igem.org/Team:Oxford/Events"><img src=
             "https://static.igem.org/mediawiki/2014/a/af/OxigemEvents.png" style="width: 100%;"></a>
             "https://static.igem.org/mediawiki/2014/a/af/OxigemEvents.png" style="width: 100%;"></a>
           </div>
           </div>
-
           <div class="Heading" style="top: 23%; left: 16%; width:18.5%;" onmouseover="iGEMEuropebig(this)" onmouseout=
+
           <div class="Heading" style="top: 24.5%; left: 16.5%; width:17%;" onmouseover="iGEMEuropebig(this)" onmouseout=
           "iGEMEuropenormal(this)" border="0">
           "iGEMEuropenormal(this)" border="0">
             <a href="https://2014.igem.org/Team:Oxford/P&P_igem_europe"><img src=
             <a href="https://2014.igem.org/Team:Oxford/P&P_igem_europe"><img src=
Line 264: Line 232:
     <div style="position:fixed;bottom:2%;left:5%;width:90%;height:7%;background-color:#f5f5f5;border-radius:25px;opacity:0.6;">  
     <div style="position:fixed;bottom:2%;left:5%;width:90%;height:7%;background-color:#f5f5f5;border-radius:25px;opacity:0.6;">  
       <div class="Heading" style="position:fixed;bottom:3.8%; left: 7%; width:7%;max-height:6%;">
       <div class="Heading" style="position:fixed;bottom:3.8%; left: 7%; width:7%;max-height:6%;">
-
         <a href="http://www.bbsrc.ac.uk/home/home.aspx"><img src="https://static.igem.org/mediawiki/2014/3/3e/BBSRC.png"
+
         <a href="http://www.bbsrc.ac.uk/home/home.aspx" target="_blank"><img src="https://static.igem.org/mediawiki/2014/3/3e/BBSRC.png"
         style="width: 110%;"></a>
         style="width: 110%;"></a>
       </div>
       </div>
       <div class="Heading" style="position:fixed;bottom:3.8%; left: 16.5%; width:10%;max-height:6%;">
       <div class="Heading" style="position:fixed;bottom:3.8%; left: 16.5%; width:10%;max-height:6%;">
-
         <a href="http://www.biochemistry.org"><img src="https://static.igem.org/mediawiki/2014/7/75/BS.png"
+
         <a href="http://www.biochemistry.org" target="_blank"><img src="https://static.igem.org/mediawiki/2014/7/75/BS.png"
         style="width: 110%;"></a>
         style="width: 110%;"></a>
       </div>
       </div>
       <div class="Heading" style="position:fixed;bottom:3.4%; left: 29%; width:9%;max-height:6%;">
       <div class="Heading" style="position:fixed;bottom:3.4%; left: 29%; width:9%;max-height:6%;">
-
         <a href="http://www.sgm.ac.uk"><img src="https://static.igem.org/mediawiki/2014/2/20/SGM.png"
+
         <a href="http://www.sgm.ac.uk" target="_blank"><img src="https://static.igem.org/mediawiki/2014/2/20/SGM.png"
         style="width: 110%;"></a>
         style="width: 110%;"></a>
       </div>
       </div>
       <div class="Heading" style="position:fixed;bottom:4.4%; left: 40.4%; width:10%;max-height:6%;">
       <div class="Heading" style="position:fixed;bottom:4.4%; left: 40.4%; width:10%;max-height:6%;">
-
         <a href="http://www.wellcome.ac.uk"><img src="https://static.igem.org/mediawiki/2014/4/4c/WT.png"
+
         <a href="http://www.wellcome.ac.uk" target="_blank"><img src="https://static.igem.org/mediawiki/2014/4/4c/WT.png"
         style="width: 110%;"></a>
         style="width: 110%;"></a>
       </div>
       </div>
       <div class="Heading" style="position:fixed;bottom:2.7%; left: 53.5%; width:6.5%;max-height:6%;">
       <div class="Heading" style="position:fixed;bottom:2.7%; left: 53.5%; width:6.5%;max-height:6%;">
-
         <a href="http://www.deskgen.com/"><img src="https://static.igem.org/mediawiki/2014/6/65/Oxfordigem_deskgen.png"
+
         <a href="http://www.deskgen.com/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/6/65/Oxfordigem_deskgen.png"
         style="width: 110%;"></a>
         style="width: 110%;"></a>
       </div>
       </div>
       <div class="Heading" style="position:fixed;bottom:2.5%; left: 62.7%; width:9.5%;max-height:6%;">
       <div class="Heading" style="position:fixed;bottom:2.5%; left: 62.7%; width:9.5%;max-height:6%;">
-
         <a href="http://www.bioch.ox.ac.uk/"><img src="https://static.igem.org/mediawiki/2014/2/20/Oxfordigem_dept.png"
+
         <a href="http://www.bioch.ox.ac.uk/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/2/20/Oxfordigem_dept.png"
         style="width: 110%;"></a>
         style="width: 110%;"></a>
       </div>
       </div>
       <div class="Heading" style="position:fixed;bottom:3%; left: 75%; width:7%;max-height:6%;">
       <div class="Heading" style="position:fixed;bottom:3%; left: 75%; width:7%;max-height:6%;">
-
         <a href="http://www.neb.com/"><img src="https://static.igem.org/mediawiki/2014/c/c5/OxigemNeb_logo.jpg"
+
         <a href="http://www.neb.com/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/b/b1/OxigemNeb_logo.png"
         style="width: 110%;"></a>
         style="width: 110%;"></a>
       </div>
       </div>
       <div class="Heading" style="position:fixed;bottom:3%; left: 84%; width:8%;max-height:6%;">
       <div class="Heading" style="position:fixed;bottom:3%; left: 84%; width:8%;max-height:6%;">
-
         <a href="http://www.snapgene.com/"><img src="https://static.igem.org/mediawiki/2014/f/ff/Oxfordigem_snapgene.png"
+
         <a href="http://www.snapgene.com/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/f/ff/Oxfordigem_snapgene.png"
         style="width: 110%;"></a>
         style="width: 110%;"></a>
       </div>
       </div>

Latest revision as of 19:15, 2 June 2015