Team:Nankai/Characterization

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<p>What we have to do is insert the genes rhlABRI to the plasmid pBBR1MCS-2. To accomplice this, we figure out 4 steps as followed.</p>
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<p>1.Restriction endonuclease site</p>
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<p>Considering P. aeruginosa SQ6 is a wild-type strain, we retained the genes’ native promoter from the strain. Comparing the restriction sites of multiple cloning sites of the plasmid pBBR1MCS-2 and the genes rhlABRI including the promoter, we chose the restriction endonuclease sites, Hind III and Sal I.</p>
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Revision as of 19:10, 17 October 2014

Retrieved from "http://2014.igem.org/Team:Nankai/Characterization"